May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Transfect Bovine Cornea Epithelial Cells With Chitosan-Modified Adenovirus
I.-J. Wang; M.-C. Jhuang; Y.-H. Chen; L.-K. Yeh; T.-H. Yang
Author Affiliations & Notes
  • I.-J. Wang
    Ophthalmology, National Taiwan Univ Hospital, Taipei, Taiwan
  • M.-C. Jhuang
    Institution of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan
  • Y.-H. Chen
    Institution of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan
  • L.-K. Yeh
    Ophthalmology, Chang-Gung Memorial Hospital, Linko, Taiwan
  • T.-H. Yang
    Institution of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan
  • Footnotes
    Commercial Relationships I. Wang, None; M. Jhuang, None; Y. Chen, None; L. Yeh, None; T. Yang, None.
  • Footnotes
    Support NSC95-2314-B-002-149-MY3 and NSC95-2314-B-002-009
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 2729. doi:
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      I.-J. Wang, M.-C. Jhuang, Y.-H. Chen, L.-K. Yeh, T.-H. Yang; Transfect Bovine Cornea Epithelial Cells With Chitosan-Modified Adenovirus. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2729.

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Abstract

Purpose:: To investigate the possibility to increase the transfection efficiency with chitosan-linked adenovirus in bovine cornea epithelial cells.

Methods:: Adenovirus(Ad) was modified with a heterogeneous crosslinker(GMBS), and the amino group of chitosan was modified with 2-iminothiolane. Then the chitosan-SH was reacted with GMBS-Ad via thioether. The size and zeta-potential of chitosan-modified Ad were measured. HEK293 and bovine cornea epithelial cells were transfected with adenovirus and chitosan-modified Ad.

Results:: Analysis of particle size showed an average diameter of the unmodified Ad of 120.0nm, compared with 139.5nm for the chitosan-modified Ad. Zeta-potential showed -24.9±6.4 mV for the unmodified Ad, compared with 2.5±3.7 mV for the chitosan-modified Ad. When infected, HEK293 and bovine cornea epithelial cells with the unmodified Ad appeared bright fluorescence in over 90% of cells at 24 h after infection, while the chitosan-modified Ad gave no detectable fluorescence signal.

Conclusions:: The ability of adenovirus to infect cells was essentially ablated by modification of adenovirus with chitosan.

Keywords: adenovirus • cornea: epithelium 
© 2007, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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