May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Exposure of IRBP-specific CD8 Autoreactive T Cells to Low Doses of Autoantigen Preferentially Activates Functionally Suppressive T Cell Subsets
Author Affiliations & Notes
  • Y. P. Sun
    Ophthalmology & Visual Sciences, University of Louisville, Louisville, Kentucky
  • Footnotes
    Commercial Relationships Y.P. Sun, None.
  • Footnotes
    Support Supported in part by NIH grants EY014-366, EY12974, EY14599 and the grant RG3413A4 from the National Multiple Sclerosis Society
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 2836. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Y. P. Sun; Exposure of IRBP-specific CD8 Autoreactive T Cells to Low Doses of Autoantigen Preferentially Activates Functionally Suppressive T Cell Subsets. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2836.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose:: To investigate structure and functional difference between CD4 and CD8 autoreactive T cells induced the uveitogenic peptide.

Methods:: Purified CD4 and CD8 isolated from B6 mice immunized with an uveitogenic peptide, interphotoreceptor retinoid-binding protein (IRBP) 1-20, were stimulated in vitro with varying doses of immunizing peptide. The activated T cells were determined for cytokine production, expression of Foxp3, and the suppressor activity.

Results:: CD4 autoreactive T cells underwent full activation when stimulated with high- or medium- concentrations of immunizing peptide, whereas a high dose of antigenic peptide resulted in only modest activation of CD8 autoreactive T cells. Interestingly, when stimulated by a low dose (<0.1 µg/ml) of antigen or by of a high dose of antigen and a small amount of TGF-ß1, the minimally activated CD8 T cells expressed high level of Foxp3 and gained suppressor function.

Conclusions:: Autoantigen alone induces only partial rather than full activation of CD8 autoreactive T cells. Full activation of CD8 autoreactive T cells may requires additional stimulatory signals, including those delivered by cytokines produced by activated CD4 T cells. Partial activation of CD8 autoreactive T cells preferentially induces functionally suppressive T cell subsets.Supported in part by NIH grants EY014-366, EY12974, EY14599 and the grant RG3413A4 from the National Multiple Sclerosis Society.

Keywords: autoimmune disease • uveitis-clinical/animal model 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×