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H. Kim, J. Yang, I. Yun, H. Lee, I. Chung; Expression of Vegf and Stat 3 in Experimental Diabetic Rat Retina. Invest. Ophthalmol. Vis. Sci. 2007;48(13):3407.
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Diabetic retinopathy is a most important cause of adult blindness. The pathogenesis of diabetic retinopathy has not been known entirely yet. But the role of VEGF in diabetic retinopathy is getting important recently. In this experience, we investigate the possibility of relationship between STAT3 and VEGF in experimental diabetic rat retina. And the possible inhibitive effect of lipoic acid on the expression of VEGF and STAT3 was also studied in the same diabetic model.
Diabetes was induced chemically by injection of streptozotocin in 12 rats of 18 Sprague-Dawley rats. After induction of diabetes, lipoic acid was injected into the peritonium in 6 rats. So all rats were divided into 3 groups, control group(n=6), Diabetes mellitus(DM) group(n=6), lipoic acid treated group(n=6). Eye balls were removed after 8 weeks of diabetes induction. Difference of VEGF and STAT3 expression was investigated by immunohistochemistry, RT-PCR, western blot. Change of VEGF and STAT3 in lipoic acid treated group were evaluated in these experimental model.
VEGF and STAT3 expression was elevated in diabetic rat retina. Active form STAT3, phosphrylated STAT3 was also elevated. Expression of VEGF and STAT3 in lipoic acid treated group was lower than DM group.
Increased expression of STAT3, especially phosphorylated STAT3 is closely related with expression of VEGF. STAT3 has a possibility as the new target for the treatment of diabetic retinopathy. Administration of lipoic acid lowered VEGF and STAT3 expression. Lipoic acid as antioxidant may have adjunctive effect in diabetic retinopathy.
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