May 2007
Volume 48, Issue 13
ARVO Annual Meeting Abstract  |   May 2007
Economical LED Based, Real-Time, in vivo Imaging of Murine Corneal Wound Healing
Author Affiliations & Notes
  • S. Y. Ghoghawala
    University of California, Davis, California
    School of Medicine,
  • M. J. Mannis
    University of California, Davis, California
  • C. J. Murphy
    Schools of Veterinary Medicine, Medicine and Engineering, University of Wisconsin, Madison, Wisconsin
  • M. I. Rosenblatt
    University of California, Davis, California
  • R. R. Isseroff
    University of California, Davis, California
  • Footnotes
    Commercial Relationships S.Y. Ghoghawala, None; M.J. Mannis, None; C.J. Murphy, None; M.I. Rosenblatt, None; R.R. Isseroff, None.
  • Footnotes
    Support NIH-NIAMS (AR44518 to RRI), NIH-NEI (K08EY015829 to MIR and RO1EY012253-05 to CJM), NIH-NHLBI (1R01HL079012-01A1 to CJM) and Research to Prevent Blindness (JMJRPBS to MJM)
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 3473. doi:
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    • Get Citation

      S. Y. Ghoghawala, M. J. Mannis, C. J. Murphy, M. I. Rosenblatt, R. R. Isseroff; Economical LED Based, Real-Time, in vivo Imaging of Murine Corneal Wound Healing. Invest. Ophthalmol. Vis. Sci. 2007;48(13):3473.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose:: An optimal system for monitoring in vivo corneal wound healing is inexpensive, has utility for wounding and imaging, and is able to provide previews before photography. We outline such an imaging system that takes advantage of a consumer digital camera and an LED-based light source for fluorescein excitation.

Methods:: Using FVB/NJ mice, 2 mm diameter, circular, axial corneal epithelial defects were created using a crescent blade. The in vivo corneal wounds were imaged every four hours until healed using a Nikon Coolpix 5400 camera attached to a Nikon SMZ-10A stereomicroscope, with fluorescein and illumination from a 16 LED 464 nm flashlight. The wound area was calculated, and the linear regressions of the linear phase of wound healing were compared using the F-test.

Results:: The slopes of the linear regressions for the 6 trials of 4 mice/trial had an average of -52.95 µm/hr (SEM = 0.55 µm/hr) and were statistically equivalent (p>0.05). The mean of the R-squared values for the linear regressions was 0.9546 (SEM = 0.0121).

Conclusions:: The equivalent linear regressions and R-squared >0.90 suggest that the imaging system could precisely monitor the wound healing of multiple trials and of animals within each trial, respectively. Using a consumer digital camera and novel LED-based illumination, we have established a system that is economical, is used in both wounding and imaging, is operated by a single person, and is able to provide real-time previews to monitor corneal wound healing precisely.

Keywords: cornea: epithelium • wound healing • microscopy: light/fluorescence/immunohistochemistry 

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