May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Effects of Subretinal Injection of Patent Blue and Trypan Blue in Rabbits
Author Affiliations & Notes
  • V. F. Lima
    Ophthalmology, Federal University of São Paulo, São Paulo, Brazil
  • E. B. Rodrigues
    Ophthalmology, Federal University of São Paulo, São Paulo, Brazil
  • M. Maia
    Ophthalmology, Federal University of São Paulo, São Paulo, Brazil
  • F. M. Penha
    Ophthalmology, Federal University of São Paulo, São Paulo, Brazil
  • A. Principe
    Ophthalmology, Federal University of São Paulo, São Paulo, Brazil
  • E. Dib
    Ophthalmology, Federal University of São Paulo, São Paulo, Brazil
  • C. Meyer
    Ophthalmology, Federal University of São Paulo, São Paulo, Brazil
  • E. Freymuller
    Ophthalmology, Federal University of São Paulo, São Paulo, Brazil
  • M. E. Farah
    Ophthalmology, Federal University of São Paulo, São Paulo, Brazil
  • Footnotes
    Commercial Relationships V.F. Lima, None; E.B. Rodrigues, None; M. Maia, None; F.M. Penha, None; A. Principe, None; E. Dib, None; C. Meyer, None; E. Freymuller, None; M.E. Farah, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 3703. doi:https://doi.org/
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    • Get Citation

      V. F. Lima, E. B. Rodrigues, M. Maia, F. M. Penha, A. Principe, E. Dib, C. Meyer, E. Freymuller, M. E. Farah; Effects of Subretinal Injection of Patent Blue and Trypan Blue in Rabbits. Invest. Ophthalmol. Vis. Sci. 2007;48(13):3703. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: To investigate the histologic and clinical effects of subretinal injection of patent blue (PB) and trypan blue (TB) in rabbits

Methods:: Dutch-belted rabbits (n= 8) were vitrectomized followed by subretinal injection of 2.4 mg/ml PB (285 mOsm) and 1.5 mg/ml TB (312 mOsm); balanced salt solution (BSS) (300 mOsm) served as the control. Animals were examined 6, 12, and 24 hours and 14 days after the procedure by fluorescein angiography (FA) and indirect ophthalmoscopy; for retinal toxicity evaluation histologic studies were performed by light and transmission electron microscopy.

Results:: FA examination demonstrated window defects suggestive of RPE atrophy in positions of subretinal TB injection, but this was not observed following subretinal injection of PB or BSS. Histological evaluation disclosed only minimal abnormalities on the photoreceptor outer segment (POS) after subretinal injection of BSS during all follow-up. Subretinal injection of PB caused POS and photoreceptor inner segment (PIS) abnormalities 12 and 24 hours following surgery as well as outer nuclear layer (ONL) damage 14 days after surgery. Subretinal TB injection resulted in POS and PIS damage at 12 hours follow-up. The ONL damage was observed 24 h after surgery; additionally, POS, PIS, ONL and RPE abnormalities were observed 14 days following surgery after TB injection.

Conclusions:: Subretinal injection of TBinduced more significant clinical and histological damage of neurosensory retina/RPE than did PB or BSS. Future human studies are necessary to access the clinical relevance of these in vivo experiments.

Keywords: retina • photoreceptors • pathology: experimental 
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