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Y. Lu, E. Filippova, J. Heckenlively; Serum Levels of Antibodies to Carbonic Anhydrase II in Patients With Retinitis Pigmentosa. Invest. Ophthalmol. Vis. Sci. 2007;48(13):3747.
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Autoimmune anti-retinal antibodies (ARAs) are postulated to play an important role in Cancer Associated Retinopathy (CAR), Melanoma Associated Retinopathy (MAR), and CAR-like syndrome, which includes retinitis pigmentosa (RP) patients with an autoimmune exacerbation of their retinopathy from anti-retinal antibodies. Carbonic anhydrase II (CAII) has been implicated as a causative antigen in our and other investigators’ work. We have initiated investigations of CAII as reported below.
Serum samples from 59 patients diagnosed with RP were screened for antiretinal proteins activity by western blotting. Enzyme linked immunosorbent assay (ELISA) were performed to evaluate the titer of anti-CAII antibodies. Patient records were reviewed to ascertain if there was a specific clinical association with CAII antibodies.
We found ~30 kDa band in 23 (39%) out of 59 patients’ sera. As compared with normal controls (0.08±0.06), there was no significant difference in the CA II antibody titer in RP patients (0.11±0.15) (p=0.52). However, 9 (39%) out of these 23 patients show high titers (A405 > 0.110) of antibodies to CAII (0.24±0.13). No clear associated was seen between antibodies to CAII and electroretinographic dysfunction, presence of cystoid edema, retinal wrinkling, or unique visual field alterations in these 9 patients.
ARAs at 30 kDa are relatively common when testing for antibody activity in the group of RP patients who have circulating ARAs. We found that 9 out of 23 (39%) patients with high titer CAII antibody but no specific clinical association. This suggests that CA II antibody in RP patients may be less significant diagnostically. The pathogenetic role of anti-CA II antibody remains uncertain.
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