May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Receptive-Field Center-Surround Organization of V1 and V2 Neurons in 2- and 4-Week-Old Monkeys Under Barbiturate and Non-Barbiturate Anesthesia
Author Affiliations & Notes
  • J. Zheng
    College of Optometry, University of Houston-Downtown, Houston, Texas
  • B. Zhang
    College of Optometry, University of Houston-Downtown, Houston, Texas
  • C. Nakatsuka
    College of Optometry, University of Houston-Downtown, Houston, Texas
  • H. Bi
    College of Optometry, University of Houston-Downtown, Houston, Texas
  • E. L. Smith
    College of Optometry, University of Houston-Downtown, Houston, Texas
  • Y. M. Chino
    College of Optometry, University of Houston-Downtown, Houston, Texas
  • Footnotes
    Commercial Relationships J. Zheng, None; B. Zhang, None; C. Nakatsuka, None; H. Bi, None; E.L. Smith, None; Y.M. Chino, None.
  • Footnotes
    Support NIH Grant EY-08218, EY-03611, RR-07146
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 3754. doi:
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      J. Zheng, B. Zhang, C. Nakatsuka, H. Bi, E. L. Smith, Y. M. Chino; Receptive-Field Center-Surround Organization of V1 and V2 Neurons in 2- and 4-Week-Old Monkeys Under Barbiturate and Non-Barbiturate Anesthesia. Invest. Ophthalmol. Vis. Sci. 2007;48(13):3754.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: We previously reported that the RF center-surround organization of V2 neurons is immature during early development (Zhang et al, PNAS, 2005). Because the receptive-field properties of V2 neurons in neonates may be differentially influenced by the type of anesthetic/analgesic agents that are used for acute microelectrode experiments, we compared the response properties of V1 and V2 neurons at 2- and 4-weeks of age and at maturity in monkeys that were anesthetized with barbiturate or non-barbiturate agents.

Methods:: Seven infant and 6 adult macaque monkeys (Macaca mulatta) were prepared for microelectrode recording experiments. Anesthesia was maintained by continuous infusion of one of the following two anesthetic/analgesic agents: 1) Sodium Pentobarbital (Nembutal, 2 mg/kg/hr), or 2) a mixture of Propofol (4 mg/kg/hr)/Sufentanyl citrate (0.05 µg/kg/hr).

Results:: Regardless of which anesthesia was used, the average RF center size of V1 and V2 neurons in 2-week-old infants was about 2-3 times as large as those in adults. The majority of V2 units recorded in 2-week-old infants lacked RF surrounds. Surround suppression in V2 was weaker by a factor of 5-6 in 2-week-old infants and by a factor of about 2 in 4-week-old infants. The average peak-firing rate of V2 neurons was lower in 2-week-old infants by a factor of about 3.0 and by a factor of 2.0 in 4-week-old infants compared to that in adults. The temporal response properties and contrast sensitivity of V1 and V2 neurons in infant monkeys exhibited significant immaturities.

Conclusions:: The immature RF center/surround organization and substantial immaturities in other response properties of V1 and V2 neurons found in 2- and 4-week-old infants do not reflect the ‘undesirable effects’ that are unique to barbiturate anesthesia.

Keywords: visual cortex • electrophysiology: non-clinical • development 
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