Purchase this article with an account.
K. M. Nishiguchi, H. Kaneko, M. Nakamura, S. Kachi, H. Terasaki; Murine Retinal Progenitor Cells in the Ciliary Body Epithelium May Participate in Neurogenesis of the Peripheral Retina in vivo. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4081.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To study if the murine retinal progenitors present in the ciliary body epithelium (CBE) may migrate into the retina and participate in neurogenesis of the developing retina.
We injected bromodeoxyuridine (BrdU) intraperitoneally to label cells that are in the S-phase of the cell cycle in C57Bl/6J mice of various age. The eyes were collected 3 hours later and the distribution of the BrdU-positive cells in the CBE and peripheral retina were immunohistochemically analyzed in sections or flatmounts. The expression of rod photoreceptor marker rhodopsin was also studied in the flatmounts.
The proliferating BrdU-positive cells were abundunt in the CBE of post-natal day 6 (P6) C57Bl/6J mice (14.1 ± 3.5 per section: mean ± 1σ ) but rapidly decreased by 95.0% in P18 (0.7 ± 0.4 per section: mean ± 1σ ). Using flatmount technique of ciliary body and retina, we found that, at P6, majority of the BrdU-positive cells in the CBE expressed rhodopsin. Some of them had structures that were similar to the inner segments and outer segments thereby resembled mature rod photoreceptors. By P9, majority of the BrdU- and rhodopsin-double-positive cells that had outer segment-like structures aligned at the cilio-retinal border in an organized fashion where the cell bodies resided within the ciliary body while the outer segment-like structures stretched into the peripheral retina. By P12, such cells with outer segment-like structures were no longer found in the CBE. We also found that, in the developing murine retina, the pattern of rhodopsin expression within the photoreceptors and the morphology of their outer segments at the peripheral retina were different from those of the remaining retina. Rhodopsin was evenly expressed throughout the cell surface including the cell body with the outer segments being randomly directed in the former. On the contrary, in the latter, rhodopsin was expressed most predominantly in the outer segments which was radially aligned toward the optic nerve. Some of the cells double-positive for rhodopsin and BrdU in the CBE were morphologically identical to the rod photoreceptors in the peripheral retina.
Many BrdU-positive retinal progenitor cells in the CBE expressed rhodopsin. Some of them had complicated structures that closely mimiced the inner segments and outer segments seen in mature photoreceptors. These cells appeared to migrate and align at the cilio-retinal margin, where they may enter the retina to participate in the neurogenesis of the developing peripheral retina.
This PDF is available to Subscribers Only