May 2007
Volume 48, Issue 13
ARVO Annual Meeting Abstract  |   May 2007
Genetic Dissection of Retinal Pathways Using the Electroretinogram (ERG)
Author Affiliations & Notes
  • M. E. Pennesi
    Dept of Ophthalmology, Univ of California - San Francisco, San Francisco, California
  • M. M. Abd-El-Barr
    Dept of Ophthalmology,
    Baylor College of Medicine, Houston, Texas
  • S. M. Saszik
    Northwestern University, Chicago, Illinois
  • J.-J. Pang
    Dept of Ophthalmology,
    Baylor College of Medicine, Houston, Texas
  • D. E. Bramblett
    Dept of Cell Biology,
    Baylor College of Medicine, Houston, Texas
  • F. Postma
    Dept of Neurobiology, Harvard University, Boston, Massachusetts
  • D. L. Paul
    Dept of Neurobiology, Havard University, Boston, Massachusetts
  • L. J. Frishman
    School of Optometry, University of Houston, Houston, Texas
  • S. M. Wu
    Dept of Ophthalmology,
    Baylor College of Medicine, Houston, Texas
  • Footnotes
    Commercial Relationships M.E. Pennesi, None; M.M. Abd-El-Barr, None; S.M. Saszik, None; J. Pang, None; D.E. Bramblett, None; F. Postma, None; D.L. Paul, None; L.J. Frishman, None; S.M. Wu, None.
  • Footnotes
    Support NIH EY04446, EY02520, the Retina Research Foundation (Houston), the International Retinal Research Foundation, Inc. and Research to Prevent Blindness(MMA,MEP,SMW), R01 EY06671 (LJF) EY014127 HIGHWIRE EXLINK_ID="48:5:4183:1" VALUE="EY014127" TYPEGUESS="GEN" /HIGHWIRE (DLP,FP)
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 4183. doi:
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    • Get Citation

      M. E. Pennesi, M. M. Abd-El-Barr, S. M. Saszik, J.-J. Pang, D. E. Bramblett, F. Postma, D. L. Paul, L. J. Frishman, S. M. Wu; Genetic Dissection of Retinal Pathways Using the Electroretinogram (ERG). Invest. Ophthalmol. Vis. Sci. 2007;48(13):4183.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose:: To gain a greater understanding of the rod and cone pathways in mice using pathway-specific knockouts.

Methods:: Electroretinography (ERG) was used to record rod- and cone-driven signals from mice constitutively lacking connexin36 (Cx36-/-), a gap junction protein present in AII amacrine cell (AIIAC)and photoreceptor gap junctions and mice lacking the basic helix-loop-helix transcription factor (bhlhb4-/-), which exhibit greatly diminished numbers of rod bipolar cells (DBCr). We also examined mice in which Cx36 was conditionally deleted only in cone photoreceptors (Cx36-/fl;RGP/BPCre). Dark-adapted ERG responses to brief flashes over a wide range of stimulus energies, sufficient to evoke rod-driven positive and negative scotopic threshold responses (pSTR, nSTR) to weak stimuli (<0.3 rh*/rod), and cone-driven contributions to a- and b-waves with strong stimuli.

Results:: All mice had a pSTR. The nSTR was absent in Cx36-/- and Bhlhb4-/- mice and present in WT, WT mice with ganglion cell lesions and Cx36-/fl;RGP/BPCre mice. Cx36-/- and Cx36-/fl;RGP/BPCre mice had normal b-wave amplitudes for stimuli up to those producing 100 rh*/rod. Cx36-/- and Cx36-/fl;RGP/BPCre mice had decreased b-wave amplitudes in response to stimuli producing 100 to 1x104 rh*/rod, but normal b-waves for stimuli that produced >1x104 rh*/rod.

Conclusions:: Our data suggests that the nSTR originates from the AIIAC-AIIAC or AIIAC-depolarizing cone bipolar cell (DBCc) electrical synapse pathway, because neither Cx36-/- mice whichlack AIIAC gap junctions, nor Bhlhb4-/- mice, which have diminished DBCr input to AIIACs, exhibited nSTRs. However, the nSTR was present in the conditional Cx36 KO which is presumed to have normal AIIAC-AIIAC and AIIAC-DBCc electrical synapses Moreover, our results suggest that rod-cone coupling via Cx36 plays an important role in visual signaling for the midrange stimulus energy producing between 100 rh*/rod -1x104 rh*/rod. Both the constitutive and conditional Cx36KOs had decreased b-wave amplitudes for these mid-range stimuli, but normal b-waves in response to weaker stimuli likely to produce only rod-driven signals, and stronger stimuli that would also produce cone-driven signals.

Keywords: electroretinography: non-clinical • retinal connections, networks, circuitry • gap junctions/coupling 

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