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W. Shen, J. Zhang, S. C. Uhrinek, L. McMullen, T. T. Lam; Determination of Cytokines and Chemokines in the Retina After Retinal Ischemia-Reperfusion Injury in Rabbits Using a Multiplex System. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4203.
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To determine the levels of several cytokines and chemokines in the retina known to be changed after retinal ischemia-reperfusion injury in rabbits using a multiplex system.
A previously described model of retinal ischemia-reperfusion injury was induced in 16 rabbits. Expression of VEGF and several inflammatory mediators at 1 and 7 days post injury was measured by a multiplex system (Luminex) using retinal extracts. Levels of several cytokines were obtained in a single run using less than 50 µl samples.
Consistent with earlier reports (Hayashi et al., Invest Ophthalmol Vis Sci 1996; 37: 2146-56. Jo et al., Invest Ophthalmol Vis Sci 2003; 44: 4054-60), the multiplex system was able to detect elevated retinal levels of VEGF, and macrophage inflammatory protein-1-alpha (MIP-1α) at one day after the insult and sustained elevated levels of VEGF and MIP-1α at 7 days. There was no significant change in RANTES (regulated upon activation, normal T cells expressed and secreted genes) compared to normal retinas at both time points.
Our findings confirm earlier reports showing the change of these cytokines and chemokines in the retina after retinal ischemia-reperfusion injury and suggest that a multiplex system provides a rapid and convenient means to determine the relative levels of various biomolecules especially when the amount of samples are relatively small.
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