May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Determination of Cytokines and Chemokines in the Retina After Retinal Ischemia-Reperfusion Injury in Rabbits Using a Multiplex System
Author Affiliations & Notes
  • W. Shen
    Pharmacology, Bausch & Lomb Inc, Rochester, New York
  • J. Zhang
    Pharmacology, Bausch & Lomb Inc, Rochester, New York
  • S. C. Uhrinek
    Pharmacology, Bausch & Lomb Inc, Rochester, New York
  • L. McMullen
    Pharmacology, Bausch & Lomb Inc, Rochester, New York
  • T. T. Lam
    Pharmacology, Bausch & Lomb Inc, Rochester, New York
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 4203. doi:
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    • Get Citation

      W. Shen, J. Zhang, S. C. Uhrinek, L. McMullen, T. T. Lam; Determination of Cytokines and Chemokines in the Retina After Retinal Ischemia-Reperfusion Injury in Rabbits Using a Multiplex System. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4203.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: To determine the levels of several cytokines and chemokines in the retina known to be changed after retinal ischemia-reperfusion injury in rabbits using a multiplex system.

Methods:: A previously described model of retinal ischemia-reperfusion injury was induced in 16 rabbits. Expression of VEGF and several inflammatory mediators at 1 and 7 days post injury was measured by a multiplex system (Luminex) using retinal extracts. Levels of several cytokines were obtained in a single run using less than 50 µl samples.

Results:: Consistent with earlier reports (Hayashi et al., Invest Ophthalmol Vis Sci 1996; 37: 2146-56. Jo et al., Invest Ophthalmol Vis Sci 2003; 44: 4054-60), the multiplex system was able to detect elevated retinal levels of VEGF, and macrophage inflammatory protein-1-alpha (MIP-1α) at one day after the insult and sustained elevated levels of VEGF and MIP-1α at 7 days. There was no significant change in RANTES (regulated upon activation, normal T cells expressed and secreted genes) compared to normal retinas at both time points.

Conclusions:: Our findings confirm earlier reports showing the change of these cytokines and chemokines in the retina after retinal ischemia-reperfusion injury and suggest that a multiplex system provides a rapid and convenient means to determine the relative levels of various biomolecules especially when the amount of samples are relatively small.

Keywords: ischemia • cytokines/chemokines • inflammation 
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