May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Nuclear Factor Kappa B in Lens Epithelial Cell Proliferation
Author Affiliations & Notes
  • H.-J. Jin
    Laboratory of Ophthalmology & Visual Sience, The Catholic University of Korea, Seoul, Republic of Korea
  • S.-J. Lee
    Laboratory of Ophthalmology & Visual Sience, The Catholic University of Korea, Seoul, Republic of Korea
  • Y. Seomun
    Laboratory of Ophthalmology & Visual Sience, The Catholic University of Korea, Seoul, Republic of Korea
  • M.-J. Son
    Laboratory of Ophthalmology & Visual Sience, The Catholic University of Korea, Seoul, Republic of Korea
  • C.-K. Joo
    Laboratory of Ophthalmology & Visual Sience, The Catholic University of Korea, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships H. Jin, None; S. Lee, None; Y. Seomun, None; M. Son, None; C. Joo, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 4230. doi:
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    • Get Citation

      H.-J. Jin, S.-J. Lee, Y. Seomun, M.-J. Son, C.-K. Joo; Nuclear Factor Kappa B in Lens Epithelial Cell Proliferation. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4230.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: This study was performed to elucidate the role of nuclear factor-kappa B (NF-kappaB) in lens epithelial cell proliferation.

Methods:: Capsular bag were prepared from pocrine eyes and maintained in a 5% CO2 incubator. The NF-ΚB translocation was confirmed immunohistochemistry and western blot analysis. And activation of NF-ΚB was inhibited by sulfasalazine, a inhibitor of NF-ΚB. And the effect of sulfasalazine was also observed in lens epithelial cell. The effect of inhibitors was observed by phase contrast microscopy and scanning electron microscopy.

Results:: NF-ΚB was found in cytoplasm of non-proliferated lensepithalial cells. However, NF-ΚB moved to nucleus in proliferation cells, PCNA positive cells. This translocation was inhibited by treatment with sulfasalazine or NF-ΚB SN50 peptide. And also, the inhibitors blocked lens epithelial cell migration from equatorial to posterior capsule.

Conclusions:: NF-ΚB has been found to play an important role in the control of proliferation and regulate the growth of lens epithelial cells. The inhibition of cell proliferation by sulfasalazine and NF-ΚB SN50 peptide was observed in capsular bag model. This finding suggests that the regulation of NF-kappaB in lens epithelial cells be able to modulate posterior capsule opacification.

Keywords: cataract • proliferation • signal transduction 
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