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S. Sun, M. Deng, L. Gong, H. Feng, Y.-M. Xiao, D. W. Li; Differential Expression of CD9 and Sox2 in the Epithelial Cells of the Young Rabbit Lens. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4237.
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© ARVO (1962-2015); The Authors (2016-present)
Our previous studies have shown that telomerase activity is present in the central epithelial cells of the young rabbit lens epithelium (Huang et al., 2005. IOVS). To explore the possibility that the central epithelium might contain lens stem cells, we have analyzed expression patterns of numerous stem cell markers in both central and periphery epithelial cells of the young rabbit lens.
The capsular lens epithelia were dissected out for extraction of total RNA or used for immunocytochemistry. RT-PCR was used for mRNA expression of various stem cell marker genes. Immunocytochemistry was used for detection of various stem cell marker proteins.
Among the 7 different stem cell markers analyzed. Two of them, CD9 and Sox2, are strongly expressed in the central epithelium but are absent or barely detectable in the periphery lens epithelial cells. One is expressed similarly in both central and periphery lens epithelial cells. The remaining 4 markers are not expressed in either central or periphery lens epithelial cells.
The central epithelial cells of the ocular lens may contain lens stem cells.
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