May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Differential Expression of CD9 and Sox2 in the Epithelial Cells of the Young Rabbit Lens
S. Sun; M. Deng; L. Gong; H. Feng; Y.-M. Xiao; D. W. Li
Author Affiliations & Notes
  • S. Sun
    College of Life Sciences, Hunan Normal University, Changsha, China
  • M. Deng
    College of Life Sciences, Hunan Normal University, Changsha, China
  • L. Gong
    College of Life Sciences, Hunan Normal University, Changsha, China
  • H. Feng
    College of Life Sciences, Hunan Normal University, Changsha, China
  • Y.-M. Xiao
    College of Life Sciences, Hunan Normal University, Changsha, China
  • D. W. Li
    College of Life Sciences, Hunan Normal University, Changsha, China
    Department of Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
  • Footnotes
    Commercial Relationships S. Sun, None; M. Deng, None; L. Gong, None; H. Feng, None; Y. Xiao, None; D.W. Li, None.
  • Footnotes
    Support Supported by 1R01 EY15765, University of Nebraska Medical Center, and the Lotus Scholar Professorship funds from Hunan Province Government and Hunan Normal University.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 4237. doi:
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      S. Sun, M. Deng, L. Gong, H. Feng, Y.-M. Xiao, D. W. Li; Differential Expression of CD9 and Sox2 in the Epithelial Cells of the Young Rabbit Lens. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4237.

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Abstract

Purpose:: Our previous studies have shown that telomerase activity is present in the central epithelial cells of the young rabbit lens epithelium (Huang et al., 2005. IOVS). To explore the possibility that the central epithelium might contain lens stem cells, we have analyzed expression patterns of numerous stem cell markers in both central and periphery epithelial cells of the young rabbit lens.

Methods:: The capsular lens epithelia were dissected out for extraction of total RNA or used for immunocytochemistry. RT-PCR was used for mRNA expression of various stem cell marker genes. Immunocytochemistry was used for detection of various stem cell marker proteins.

Results:: Among the 7 different stem cell markers analyzed. Two of them, CD9 and Sox2, are strongly expressed in the central epithelium but are absent or barely detectable in the periphery lens epithelial cells. One is expressed similarly in both central and periphery lens epithelial cells. The remaining 4 markers are not expressed in either central or periphery lens epithelial cells.

Conclusions:: The central epithelial cells of the ocular lens may contain lens stem cells.

Keywords: cataract • gene/expression • aging 
© 2007, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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