May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Dissection of the Promoter Region of the Gene Encoding the Regulatory A-alpha Subunit of the Protein Phosphatase-2a
H.-G. Chen; J. Qin; Q. Yan; J.-P. Liu; W.-B. Liu; Y. Liu; D. W.-C. Li
Author Affiliations & Notes
  • H.-G. Chen
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
    College of Life Sciences, Hunan Normal University, Changsha, China
  • J. Qin
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
  • Q. Yan
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
    College of Life Sciences, Hunan Normal University, Changsha, China
  • J.-P. Liu
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
  • W.-B. Liu
    College of Life Sciences, Hunan Normal University, Changsha, China
  • Y. Liu
    College of Life Sciences, Hunan Normal University, Changsha, China
  • D. W.-C. Li
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
    College of Life Sciences, Hunan Normal University, Changsha, China
  • Footnotes
    Commercial Relationships H. Chen, None; J. Qin, None; Q. Yan, None; J. Liu, None; W. Liu, None; Y. Liu, None; D.W. Li, None.
  • Footnotes
    Support Supported by 1R01 EY15765, University of Nebraska Medical Center, and the Lotus Scholar Professorship funds from Hunan Province Government and Hunan Normal University.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 4239. doi:
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      H.-G. Chen, J. Qin, Q. Yan, J.-P. Liu, W.-B. Liu, Y. Liu, D. W.-C. Li; Dissection of the Promoter Region of the Gene Encoding the Regulatory A-alpha Subunit of the Protein Phosphatase-2a. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4239.

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Abstract

Purpose:: Protein serine/threonine phosphatases play important role in regulation gene expression, cell proliferation, differentiation, and apoptosis. The protein serine/threonine phosphatase-2A (PP-2A) is one of the major serine/threonine phosphatases in the ocular tissues. The function of PP-2A is largely regulated by the two families of regulatory subunits: A and B. In the present study, we have cloned the promoter region of the A regulatory subunit (alpha form) and dissected its regulatory regions.

Methods:: Bioinformatics and PCR were used for the cloning of the promoter region (1954 bp-named A1) of PP-2A-Aalpha. This promoter region was sequentially deleted into 4 fragments named A2 (1526), A3 (1106), A4 (773 bp) and A5 (677 bp). In vitro mutagenesis was used to conduct the point mutation to identify the cis-elements in the A5 region. Different promoter fragments and point mutation mutants were linked to luciferase reporter gene for their activity analysis in rabbit lens epithelial cells.

Results:: Reporter gene activity assays reveal that there exists a repressor between 1954 bp and 1106 bp. Within the first 677 bp (A5) of the promoter region, SP-1 plays a vital role to maintain expression from the PP-2A-Aalpha promoter. In contrast, AP-2 seems to act as a repressor in this region (A5).

Conclusions:: Multiple cis-regulatory elements are present in the promoter region of PP-2A-Aalpha. Among these elements, SP-1 and AP-2 play important roles.

Keywords: transcription • gene mapping • signal transduction 
© 2007, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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