May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Effect of Tetrandrine on Fibrogenic Behaviors of Cultured Human Subconjunctival Fibroblasts
Author Affiliations & Notes
  • A. Kitano
    Ophthalmology, Wakayama Med. Univ., Wakayama, Japan
  • O. Yamanaka
    Ophthalmology, Wakayama Med. Univ., Wakayama, Japan
  • S. Saika
    Ophthalmology, Wakayama Med. Univ., Wakayama, Japan
  • K. Ikeda
    Anatomy, Osaka City Univ., Osaka, Japan
  • Y. Okada
    Ophthalmology, Wakayama Med. Univ., Wakayama, Japan
  • K. Shirai
    Ophthalmology, Wakayama Med. Univ., Wakayama, Japan
  • Y. Ohnishi
    Ophthalmology, Wakayama Med. Univ., Wakayama, Japan
  • Footnotes
    Commercial Relationships A. Kitano, None; O. Yamanaka, None; S. Saika, None; K. Ikeda, None; Y. Okada, None; K. Shirai, None; Y. Ohnishi, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 4280. doi:
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    • Get Citation

      A. Kitano, O. Yamanaka, S. Saika, K. Ikeda, Y. Okada, K. Shirai, Y. Ohnishi; Effect of Tetrandrine on Fibrogenic Behaviors of Cultured Human Subconjunctival Fibroblasts. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4280.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: To examine the effect of tetrandrine on fibrogenic behaviors of cultured human subconjunctival fibroblasts (hSCFs). Tetrandrine is a bioactive constituent of an herbal medicine.

Methods:: Confluent cultures of hSCFs were treated with tetrandrine (0 - 5.0 micro g/ml). Its effects on expression of a-smooth muscle actin, collagen I and III, and fibronectin were investigated by using immunocytochemistry, western blotting, enzyme-immunoassay or real-time RT-PCR. Tetrandrin's effect on cell migration or proliferation was determined by in vitro wound closure model in a cell monolayer or by Alamar blue assay, respectively. Effect of tetrandrine on Smad2 phosphorylation and Smad7 expression was examined by immunocytochemistry and western blotting.

Results:: Adding tetrandrine suppressed expression of collagens and fibronectin at mRNA or protein level and cell migration, but not cell proliferation. Treatment of cells with tetrandrine up-regulated expression of Smad7, an inhibitory Smad, and reduced nuclear translocation of phospho-Smad2 in cells. Control cells showed a peak of Smad2 acticvation at 0.5 hr.

Conclusions:: Tetrandrine inhibits fibrogenic behaviors of cultured hSCFs. The mechanism of action might include that this compound induce Smad7 that resulted in supprssion of Smad2 signal.

Keywords: conjunctiva • extracellular matrix • growth factors/growth factor receptors 
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