May 2007
Volume 48, Issue 13
ARVO Annual Meeting Abstract  |   May 2007
Corneal Endothelial Cell Sample Profile on Specular Microscopy With Reliability and Reproducibility
Author Affiliations & Notes
  • F. C. Abib
    Ophthalmology, Federal University of Parana, Curitiba, Brazil
  • A. R. C. Schaefer
    Ophthalmology, University of São Paulo, São Paulo, Brazil
  • Tâ. M. C. Schaefer
    Ophthalmology, University of São Paulo, Curitiba, Brazil
  • R. Godois
    Biology, Playmed, Cianorte, Brazil
  • R. A. Laing
    Bio-Optics, Inc., Sarasota, Florida
  • Footnotes
    Commercial Relationships F.C. Abib, P, P; A.R.C. Schaefer, None; T.M.C. Schaefer, None; R. Godois, P, P; R.A. Laing, P, P.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 4286. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      F. C. Abib, A. R. C. Schaefer, Tâ. M. C. Schaefer, R. Godois, R. A. Laing; Corneal Endothelial Cell Sample Profile on Specular Microscopy With Reliability and Reproducibility. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4286.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose:: To determine the corneal endothelial sample profile in specular microscopy with reliability and reproducibility.

Methods:: A retrospective study was performed of 100 right eyes of patients who had undergone corneal specular microscopy, with different clinical situations, in March 2006 in a private clinic. The Bio-Optics specular microscopy was used with the Bambi 2000 Plus software. To compute the sample size we used the software package, CELLS ANALYZER - Corneal Endothelial Statistical LabPat Rec with planned reliability degree (RD) 95%. The sampling process of acquiring endothelial images and counting those cell images were stoped when the calculated relative error (RE) < 0.05. Endothelial samples were evaluated in relation to calculated sample size, considered number of endothelial images, and cell number of the specular microscopy exams.

Results:: The sex distribution was 64 (64%) of the eyes of female patients and 36(36%) of male patients. The minimum age was 16 years, the maximum 96 years and the average 51±19 years. The smallest computed sample size was 57 cells, the largest 315 cells and the average was 138±45 cells; The smallest endothelial sampling had 1 image, the largest 7 images and the average 2.35±1.05 images.

Conclusions:: To make corneal specular microscopy with reliability and reproducibility (RD 95% and RE<0.05) the sampling process needed 1 to 7 (2.35±1) different images and a large number of endothelial cells counted in different areas of corneal endothelial mosaics.

Clinical Trial:: BANPESQ - UFPR - BRAZIL

Keywords: cornea: endothelium • clinical (human) or epidemiologic studies: biostatistics/epidemiology methodology • microscopy: confocal/tunneling 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.