May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Expression of HLA-DR on the Ocular Surface of Patients With Severe Ocular Burns
Author Affiliations & Notes
  • R. Navarre
    Poitiers University Hospital, Poitiers, France
    Ophthalmology,
  • J.-J. Gicquel
    Poitiers University Hospital, Poitiers, France
    Ophthalmology,
  • A. Coulon
    Poitiers University Hospital, Poitiers, France
    Pathology,
  • M. Langman
    Poitiers University Hospital, Poitiers, France
    Ophthalmology,
  • J. Gombert
    Poitiers University Hospital, Poitiers, France
    Immunology,
  • P. Levillain
    Poitiers University Hospital, Poitiers, France
    Pathology,
  • P. Dighiero
    Poitiers University Hospital, Poitiers, France
    Ophthalmology,
  • Footnotes
    Commercial Relationships R. Navarre, None; J. Gicquel, None; A. Coulon, None; M. Langman, None; J. Gombert, None; P. Levillain, None; P. Dighiero, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 4308. doi:
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      R. Navarre, J.-J. Gicquel, A. Coulon, M. Langman, J. Gombert, P. Levillain, P. Dighiero; Expression of HLA-DR on the Ocular Surface of Patients With Severe Ocular Burns. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4308.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: To measure by the Impression Cytology (IC) technique the expression of HLA-DR by the conjunctival epithelium from 0 to 2 years after severe ocular burns, the cytological modifications of the conjunctival surface according to the Nelson’s classification and their correlation.

Methods:: 24 patients (24 eyes) who presented with severe ocular burns underwent IC and were compared with 18 healthy eyes. HLA-DR expression was studied in flow cytometry as well as the conjunctival histology evaluated with the Nelson’s Classification.

Results:: There was a significant upregulation of the expression of HLA-DR compared to the healthy population at 0 month (p<0.001), 2 months (p<0.001), 6 months (p<0.001), 12 months (p=0.019), 18 months (p=0.0171) and 24 months (p=0.01766) after burn. A significant difference was found between the Grade of Nelson in the pathological population and those of the healthy population at 0 month (p<0.001) and 2 months (p=0.0157). HLA-DR upregulation was significantly correlated with the Nelson's Grades between 0 month (R = 075, p < 0.0001) and 6 months (R = 0.61, p = 0.0001).

Conclusions:: IC technique is a state of the art tool for following-up ocular surface inflammation after severe ocular burns. It may help in taking decisions regarding iterative amniotic membrane grafting and ocular surface reconstruction surgery.

Keywords: cornea: clinical science • immunomodulation/immunoregulation • conjunctiva 
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