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R. Navarre, J.-J. Gicquel, A. Coulon, M. Langman, J. Gombert, P. Levillain, P. Dighiero; Expression of HLA-DR on the Ocular Surface of Patients With Severe Ocular Burns. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4308.
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© ARVO (1962-2015); The Authors (2016-present)
To measure by the Impression Cytology (IC) technique the expression of HLA-DR by the conjunctival epithelium from 0 to 2 years after severe ocular burns, the cytological modifications of the conjunctival surface according to the Nelson’s classification and their correlation.
24 patients (24 eyes) who presented with severe ocular burns underwent IC and were compared with 18 healthy eyes. HLA-DR expression was studied in flow cytometry as well as the conjunctival histology evaluated with the Nelson’s Classification.
There was a significant upregulation of the expression of HLA-DR compared to the healthy population at 0 month (p<0.001), 2 months (p<0.001), 6 months (p<0.001), 12 months (p=0.019), 18 months (p=0.0171) and 24 months (p=0.01766) after burn. A significant difference was found between the Grade of Nelson in the pathological population and those of the healthy population at 0 month (p<0.001) and 2 months (p=0.0157). HLA-DR upregulation was significantly correlated with the Nelson's Grades between 0 month (R = 075, p < 0.0001) and 6 months (R = 0.61, p = 0.0001).
IC technique is a state of the art tool for following-up ocular surface inflammation after severe ocular burns. It may help in taking decisions regarding iterative amniotic membrane grafting and ocular surface reconstruction surgery.
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