May 2007
Volume 48, Issue 13
ARVO Annual Meeting Abstract  |   May 2007
Structural Factors Mediating Scleral Stiffness
Author Affiliations & Notes
  • D. S. Schultz
    UCSF, San Francisco, California
    Orthopedic Surgery,
    Mechanical Engineering, University of California at Berkeley, Graduate Student Researcher, California
  • J. M. Stewart
    UCSF, San Francisco, California
  • Footnotes
    Commercial Relationships D.S. Schultz, None; J.M. Stewart, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 4420. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      D. S. Schultz, J. M. Stewart; Structural Factors Mediating Scleral Stiffness. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4420.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose:: To quantify structurally relevant constituents within sclera and determine their impact on mechanical stiffness.

Methods:: Posterior strips approximately 6 mm by 25 mm were harvested from 20 pig and 20 human eyes and stored frozen (-20 °C). Mechanical stiffness was determined via quasi-static uniaxial tensile tests by calculating the tangent modulus at a prescribed near-physiologic stress (0.8MPa). Adjacent globe tissue was apportioned for each of five assays: pepsin soluble collagen, acid soluble collagen, soluble crosslink density, elastin content and glycosaminoglycan content.

Results:: Preliminary results for porcine sclera (n=6) indicate stiffness values of 47.0 MPa (stdev = 10.7 MPa), water content of 68.0% (stdev = 1.9%) and soluble collagen content of 37.9% (stdev = 9.7%). It is expected that variance in stiffness values will be dependent on collagen content and crosslink density. In contrast, water and glycosaminoglycan content are unlikely to influence a quasi-static measure of stiffness. An analysis of variance will be used to determine the dependence of stiffness on each tissue constituent.

Conclusions:: Variation in collagen content and crosslink density will likely have a statistically significant impact on the variation in mechanical stiffness. Elastin is likely to have a lesser role influencing stiffness, while water content and glycosaminoglycan content are expected to be statistically insignificant factors.

Keywords: myopia • sclera • refractive error development 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.