May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Immunohistochemical Identification of IL-1B in Equine Fungal Keratitis
Author Affiliations & Notes
  • P. A. Moore
    College Vet Med Univ Georgia, Athens, Georgia
    Dept Small Animal Med/Surg,
  • S. D. Boveland
    College Vet Med Univ Georgia, Athens, Georgia
    Department of Pathology,
  • J. Mysore
    College Vet Med Univ Georgia, Athens, Georgia
    Department of Pathology,
  • U. Dietrich
    College Vet Med Univ Georgia, Athens, Georgia
    Dept Small Animal Med/Surg,
  • C. Jarret
    College Vet Med Univ Georgia, Athens, Georgia
    Department of Anatomy and Radiology,
  • K. P. Carmichael
    College Vet Med Univ Georgia, Athens, Georgia
    Department of Pathology,
  • Footnotes
    Commercial Relationships P.A. Moore, None; S.D. Boveland, None; J. Mysore, None; U. Dietrich, None; C. Jarret, None; K.P. Carmichael, None.
  • Footnotes
    Support The Veterinary Ophthalmology Research Fund and The Clinical Research Fund – The University of Georgia
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 4736. doi:https://doi.org/
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    • Get Citation

      P. A. Moore, S. D. Boveland, J. Mysore, U. Dietrich, C. Jarret, K. P. Carmichael; Immunohistochemical Identification of IL-1B in Equine Fungal Keratitis. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4736. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: Identify the role of IL-1B in the pathogenesis of equine fungal keratitis and purulonecrotic keratitis

Methods:: Paraffin-embedded normal (n=3), purulonecrotic (n=10) and fungal keratitis (n=9) corneal samples were evaluated immunohistochemically for IL-IB. The number of immunoreactive inflammatory cells (macrophages and neutrophils) was counted in 10 high power fields and the staining intensity (SI) scored from 1-4. A student’s t-test was performed to compare the number of immunoreactive inflammatory cells (IC) and SI between the all three groups. Significance was set at p≤ 0.05.

Results:: The mean IC cout for the three groups were 2.86 for the purulonecrotic, 4.67 for the fungal keratitis and 0 for the control samples. The mean SI for the three groups were 2.10 for the purulonecrotic, 2.11 for the fungal keratitis and 0 for the control samples. The number of IC, and SI was significantly higher for IL-1B in the purulonecrotic and fungal keratitis samples when compared to the controls. Three was no significant difference in the number of IC and SI between purulonecrotic and fungal keratitis samples.

Conclusions:: IL-1B is expressed in equine purulonecrotic and fungal keratitis, and may play are role in their pathogenesis.

Keywords: keratitis • fungal disease • immunohistochemistry 
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