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A. H. Wolf, I. W. Reiniger, U. C. Schaller, D. Kook, A. J. Mueller, A. Kampik, U. C. Welge-Luessen; TGF-Beta Induces Osteopontin in Uveal Melanoma Cells. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4760.
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TGF-beta has been shown to have a tumor promoting effect in uveal melanoma. In carcinomas TGF-beta is thought to promote the ability to metastasize. Osteopontin (OPN) is a promising tumor marker for uveal melanoma and other malignancies. Aim of our study was to investigate the coherence of the TGF-beta and the OPN in uveal melanoma cells.
Cells of 5 untreated uveal melanoma were grown until confluency. For specification of cells, immunohistological staining against S100, HMB-45 and OPN was performed. Confluent cells were then treated with TGF-beta at 1,0 mg/ml for 6 to 48 hrs. Concentrations of OPN were measured thereafter by quantititive RT-PCR.
All grown cells showed positive staining for S 100, HMB-45 and OPN. Concentrations of OPN increased markedly in 4 out of 5 uveal melanomas when treated with TGF-beta. Highest increase of OPN was found in cells treated for 24 hrs at 1mg/ml TGF-beta. At this concentration we found an up to 3-fold increase of OPN.
TGF-beta induces OPN in uveal melanoma cells. Thus, elevated levels of OPN as found in patients with metastastatic uveal melanoma might be induced by increased TGF-beta. Our results point out the need to further investigate the role of TGF-beta in the genesis of uveal melanoma.
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