Bevacizumab Binding to Human Sclera Post Topical Application

Abstract

 

Purpose:

 

Bevacizumab (Avastin®, Genentech, California, USA), is an anti-angiogenesis medication used for cancer treatment and may have potential in modulating the fibrovascular response post trabeculectomy. This study will evaluate the amount of retained bevacizumab on scleral tissue after topical application.

 

Methods:

 

Scleral bevacizumab concentrations were determined using an enzyme-linked immunosorbent assay (ELISA) with the wells coated with avidin. Biotinylated bevacizumab was used as the primary antibody. Goat anti-human IgG conjugated to horseradish peroxidase as the secondary antibody. Four concentrations of biotinylated bevacizumab (1.25 mg, 0.6 mg, 0.3 mg and 0.125 mg per 0.05ml) as standards . The supernatant of human scleral tissue was treated with the biotinylated bevacizumab for 30 minutes prior to performing the ELISA. Concentrations of each supernatant tissue group were determined as a comparison to the standards.

 

Results:

 

 

 

Conclusions:

 

Bevacizumab is retained in scleral tissue after topical application and may have a role in treating fibrovascular diseases of the ocular surface. Further studies are needed to better delineate optimal delivery technique and dosing.

 

Keywords: immunohistochemistry • sclera