May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Evaluation of a Strabismus Susceptibility Locus on Chromosome 7p in Japanese
Author Affiliations & Notes
  • H. Fujiwara
    Ophthalmology, Okayama Univ Medical School, Okayama-shi, Japan
  • T. Matsuo
    Ophthalmology, Okayama Univ Medical School, Okayama-shi, Japan
  • S. Shaaban
    Ophthalmology, Okayama Univ Medical School, Okayama-shi, Japan
  • S. Hasebe
    Ophthalmology, Okayama Univ Medical School, Okayama-shi, Japan
  • T. Furuse
    Ophthalmology, Okayama Univ Medical School, Okayama-shi, Japan
  • R. Kono
    Ophthalmology, Okayama Univ Medical School, Okayama-shi, Japan
  • H. Ohtsuki
    Ophthalmology, Okayama Univ Medical School, Okayama-shi, Japan
  • N. Kamatani
    Internal Medicine, Tokyo Women's Medical University, Tokyo, Japan
  • Footnotes
    Commercial Relationships H. Fujiwara, None; T. Matsuo, None; S. Shaaban, None; S. Hasebe, None; T. Furuse, None; R. Kono, None; H. Ohtsuki, None; N. Kamatani, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 4857. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      H. Fujiwara, T. Matsuo, S. Shaaban, S. Hasebe, T. Furuse, R. Kono, H. Ohtsuki, N. Kamatani; Evaluation of a Strabismus Susceptibility Locus on Chromosome 7p in Japanese. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4857.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose:: We examined whether the 7p22.1 region reported in the United States would be also a candidate locus for our Japanese pedigrees with strabismus.

Methods:: According to the method approved by the Ethical Committee of Okayama University Medical School, genomic DNA was extracted from blood samples obtained from strabismic patients and their family members. Using an ABI310 Genetic AnalyzerTM, genotypes were determined with microsatellite markers in the 7p22.1 region. Non-parametric linkage (NPL) scores were calculated by genetic statistical analysis with a computer program (GenehunterTM), and the values were compared with those reported in the American pedigrees.

Results:: Blood samples were collected from members of 52 pedigrees with family history of exotropia or esotropia. In 52 pedigrees, the NPL scores (p values) were 0.44 (0.31) at D7S531, 0.50 (0.29) at D7S517, 1.71 (0.03) at D7S513, 1.24 (0.09) at D7S507, 0.82 (0.18) at D7S493, and 1.23 (0.42) at D7S516, indicating probable linkage of this chromosomal locus with strabismus in the 52 Japanese pedigrees. Twenty (38%) of the 52 pedigrees showed an NPL score over 1.00 in the 7p22.1 region. We focused on these pedigrees as a subgroup and calculated the NPL scores. In the subgroup of 20 pedigrees, NPL scores (p values) were 4.36(3.43x10-6) at D7S531, 4.68(5.77x10-7) at D7S517, 4.92(9.88x10-8) at D7S513, 4.66(5.77x10-7) at D7S507, 4.00(1.70x10-5) at D7S493, and 3.47(1.88x10-4) at D7S516, indicating significant linkage of this chromosomal locus with strabismus in these 20 Japanese pedigrees.

Conclusions:: The results suggested multiple strabismus-related loci in the Japanese pedigrees and one strabismus-related locus located in the 7p22.1 region.

Keywords: strabismus • gene mapping • linkage analysis 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×