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S. Jeong, Y. S. Yu, J. Kim, K.-H. Park, J. Kim, K.-W. Kim; VEGF-Mediated Blood-Retinal Barrier Breakdown in Experimental Models of Diabetic Retinopathy Is Closely Linked to Renin-Angiotensin System. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4972.
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To evaluate the coordinated action of renin-angiotensin system and vasopermeable factor on blood-retinal barrier breakdown of diabetic retinopathy
In experimental mice models of diabetic retinopathy with or without intravitreal injection of ACE inhibitor, retinal vascular leakage was evaluated through intravenous injection of FITC-dextran. Expression of angiotensin II (ANG II), angiotensin II receptor type 1 (ATR1) & 2 (ATR2), and VEGF were evaluated by immunohistochemistry and Western blotting. Tight junction proteins such as ZO-1, ZO-2, and occludin were checked as well. In Human retinal endothelial cell (HREC), VEGF-induced tight junction protein expression was measured with or without ACE inhibitor treatment
With ACE inhibitor, retinal vascular leakage was blocked, which was followed by decrease of ANG II, ATR1, ATR2, and VEGF expression and recovery of tight junction protein expression in the retina of diabetic mice. Also, VEGF-induced decrease of tight junction proteins was blocked by ACE inhibitor.
Our data suggests that blockade of the renin-angiotensin system can confer the prevention of VEGF mediated blood-retinal barrier breakdown in experimental models of diabetic retinopathy.
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