May 2007
Volume 48, Issue 13
ARVO Annual Meeting Abstract  |   May 2007
Inflammatory Markers in Diabetic Rat Retina
Author Affiliations & Notes
  • E. Agardh
    Malmo University Hospital, Malmo, Sweden
    Ophthalmology/Department of Clinical Sciences,
  • C. Gustavsson
    Malmo University Hospital, Malmo, Sweden
    Ophthalmology/Department of Clinical Sciences,
  • C.-D. Agardh
    Malmo University Hospital, Malmo, Sweden
    Endocrinology/Department of Clinical Sciences,
  • Footnotes
    Commercial Relationships E. Agardh, None; C. Gustavsson, None; C. Agardh, None.
  • Footnotes
    Support Lund University, the Swedish Diabetes Federation, the Järnhardt Foundation, Malmö University Hospital Foundation, the Foundation for Visually Impaired in Former Malmöhus Län, and the Skane County Coun
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 4979. doi:
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    • Get Citation

      E. Agardh, C. Gustavsson, C.-D. Agardh; Inflammatory Markers in Diabetic Rat Retina. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4979.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose:: To evaluate the expression of inflammatory markers in diabetic rat retina exposed to ischemia-reperfusion injury.

Methods:: Diabetes was induced in female Wistar rats by an i.p. injection of streptozotocin. Eyes from diabetic and non-diabetic rats were exposed to retinal ischemia by ligation of the optic nerve and vessels behind the left eye bulb for 45 minutes, followed by a reperfusion for 24 hours. Retinas unexposed to ischemia-reperfusion injury from diabetic rats were compared to retinas from non-diabetic rats as well. Expressions of the inflammatory markers caspase-1, TNF-α, I-6, I-1ß, P-selectin, VCAM-1, and ICAM-1 were analyzed with real-time RT-PCR and related to the endogenous control cyclophilin B.

Results:: Diabetes increased the expressions of caspase-1 (2.8-fold, p=0.046), VCAM-1 (2.6-fold, p=0.027), and ICAM-1 (2.2-fold, p=0.016) in non-ischemic retinas. Ischemia/reperfusion increased the expressions in both non-diabetic and diabetic eyes of TNF-α 4.9-fold, (p=0.012) and 4.0-fold, (p=0.017), respectively, IL-1ß 8.9-fold, (p=0.017) and 26.9-fold, (p=0.012), respectively, ICAM-1 2.6-fold, (p=0.025) and 2.0-fold, (p=0.017), respectively and IL-6 5.6-fold, (p=0.012) and 22.2-fold, (p=0.012), respectively. The expressions of caspase-1, P-selectin and VCAM-1 were unchanged.Conclusion Diabetes triggers an increased expression of several inflammatory markers in rat retina, and ischemia-reperfusion injury induces considerably higher levels of expression.

Keywords: diabetic retinopathy • gene/expression • cytokines/chemokines 

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