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N. Kuno, F. Takamatsu, Y. Tabata; The Synergistic Protective Effect of Subretinal Transplantation of Adipose-Derived Stromal Cells and Brain Derived Neurotrophic Factor-Impregnated Gelatin Microspheres on Retinal Function in Royal College of Surgeons Rat. Invest. Ophthalmol. Vis. Sci. 2007;48(13):5082.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the protective effect of subretinal transplantation of adipose-derived stromal cells (ADSCs) and/or brain derived neurotrophic factor (BDNF)-impregnated cross-linked gelatin microspheres against photoreceptor degeneration in Royal College of Surgeons (RCS) rats.
ADSCs derived from homozygote EGFP transgenic rats and/or BDNF-impregnated cross-linked acidic gelatin microspheres (MS-BDNF) were transplanted at subretinal space in RCS rats (postnatal 3 weeks). Electroretinograms (ERGs) were recorded at 2, 4, 8 and 12 weeks after subretinal transplantation. At the end of the experiment, retinas were prepared for light microscopy or immunocytochemical staining with antibodies to rod opsin and EGFP.
Amplitude of a-wave and b-wave were disappeared in rats received subretinal injection of vehicle (PBS) at 4 weeks. In the ADSCs, MS-BDNF, and combination of ADSCs and MS-BDNF transplanted eyes, amplitudes of a- and b-wave were remained at 8 weeks after transplantation. The remaining amplitudes at 8 week after transplantation against pre-transplantation in rats received MS-BDNF was higher than in rats received ADSCs, on the other hand, transplantation of combination of ADSCs and MS-BDNF has shown synergistic protective effect. In histological study, photoreceptors were well preserved in three groups excepting vehicle group. Although ADSCs transplanted were survived at subretinal space for 12 weeks, transdifferentiation to rod photoreceptors was not observed by immunocytochemical studies.
These results suggest that subretinal transplantation of combination of ADSCs and MS-BDNF might be promising protection for retinal degeneration.
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