May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Subretinal Transplantation of Purifed Cone Precursors in Mouse Cone Degeneration Mutants
Author Affiliations & Notes
  • H. Cheng
    Opthalmology and Visual Sciences, Univ of Michigan-Kellogg Eye Ctr, Ann Arbor, Michigan
  • J. Yao
    Opthalmology and Visual Sciences, Univ of Michigan-Kellogg Eye Ctr, Ann Arbor, Michigan
  • E. Filippova
    Opthalmology and Visual Sciences, Univ of Michigan-Kellogg Eye Ctr, Ann Arbor, Michigan
  • B. Chang
    Jackson Laboratory, Bar Harbor, Maine
  • J. Heckenlively
    Opthalmology and Visual Sciences, Univ of Michigan-Kellogg Eye Ctr, Ann Arbor, Michigan
  • A. Swaroop
    Opthalmology and Visual Sciences, Univ of Michigan-Kellogg Eye Ctr, Ann Arbor, Michigan
  • Footnotes
    Commercial Relationships H. Cheng, None; J. Yao, None; E. Filippova, None; B. Chang, None; J. Heckenlively, None; A. Swaroop, None.
  • Footnotes
    Support NIH (EY11115, EY07003), Foundation Fighting Blindness (FFB), Research to Prevent Blindness (RPB), and Training Program in Organogenesis (T-32-HD007505 HIGHWIRE EXLINK_ID="48:5:5087:1" VALUE="HD007505" TYPEGUESS="GEN" /HIGHWIRE )
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 5087. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      H. Cheng, J. Yao, E. Filippova, B. Chang, J. Heckenlively, A. Swaroop; Subretinal Transplantation of Purifed Cone Precursors in Mouse Cone Degeneration Mutants. Invest. Ophthalmol. Vis. Sci. 2007;48(13):5087.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose:: Post-mitotic rod precursors are able to integrate within the outer nuclear layer by subretinal transplantion and at least partially rescue rod function in rod degeneration models. Our goal is to test whether cone precursors are able to integrate into the degenerated retina and rescue cone function.

Methods:: GFP positive cone precursors were purified from the Nrl-Ko-GFP mice by FACS analysis, and transplanted, at different time-points, into the subretinal space of the Crx::Nr2e3/WT (rod-only retina) and cone degenerative mouse models (Cnga3, Gnat2, and Pde6C mutants). ERGs were recorded to evaluate retinal function. Histology and immunohistochemistry were also performed.

Results:: Cone precursors from the Nrl-ko-GFP mice, collected at postnatal day 2-6, were able to undergo terminal differentiation in mutant retina when injected at P2-10 before retinal degeneration. Photopic ERGs showed improvement in the cone degenerative mouse (Cnga3 mutant), but not in the Crx::Nr2e3/WT mice. Immunohistochemistry revealed that the orientation of transplanted photoreceptors were reversed in the Crx::Nr2e3/WT mice and did not seem to form normal synapse with bipolar cells. Subretinal transplantations of GFP positive cells in adult degenerating retina and other cone degenerative mouse mutants (Pde6C and Gnat2) are in progress.

Conclusions:: Our data suggest a possible (at least partial) rescue of cone phenotype in mouse models showing cone degeneration.

Keywords: photoreceptors • transplantation • retinal connections, networks, circuitry 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×