May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Il-10 Gene Polymorphism in Patients With Toxoplasmic Retinochoroiditis
Author Affiliations & Notes
  • C. A. Cordeiro
    Uveitis, Hospital Sao Geraldo - UFMG, Belo Horizonte, Brazil
  • P. R. Moreira
    Instituto de Ciências Biológicas - UFMG, Belo Horizonte, Brazil
  • W. O. Dutra
    Instituto de Ciências Biológicas - UFMG, Belo Horizonte, Brazil
  • A. L. Teixeira
    Instituto de Ciências Biológicas - UFMG, Belo Horizonte, Brazil
  • W. R. Campos
    Uveitis, Hospital Sao Geraldo - UFMG, Belo Horizonte, Brazil
  • F. Oréfice
    Uveitis, Hospital Sao Geraldo - UFMG, Belo Horizonte, Brazil
  • Footnotes
    Commercial Relationships C.A. Cordeiro, None; P.R. Moreira, None; W.O. Dutra, None; A.L. Teixeira, None; W.R. Campos, None; F. Oréfice, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 5188. doi:
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      C. A. Cordeiro, P. R. Moreira, W. O. Dutra, A. L. Teixeira, W. R. Campos, F. Oréfice; Il-10 Gene Polymorphism in Patients With Toxoplasmic Retinochoroiditis. Invest. Ophthalmol. Vis. Sci. 2007;48(13):5188.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: Experimental data has demonstrated a relevant role for IL-10 in the modulation of acute ocular toxoplasmosis in murines. Therefore, we aim to investigate the possible association between IL-10 (-1082G/A) polymorphism and toxoplasmic retinochoroiditis in humans.

Methods:: Cross-sectional study which included one hundred Brazilian patients meeting well-defined criteria for the diagnosis of toxoplasmic retinochoroiditis and fifty age and gender matched control subjects with no signs of ocular toxoplasmosis in whom anti-toxoplasma antibody analysis revealed positive immunoglobulin G titers. Genomic DNA was obtained from oral swabs of all subjects and amplified using the polymerase chain reaction (PCR) with specific primers flanking the locus -1082 of IL10. PCR products were submitted to restriction endonuclease digestion and analyzed by polyacrylamide gel electrophoresis, to distinguish alleles G and A of the IL10 gene, allowing for the determination of the genotypes and detection of the polymorphism.

Results:: There were no statistically significant associations among -1082A allele and the occurrence of toxoplasmic retinochoroiditis (P = 0.80; x² = 0,065). In addition, subgroup analysis considering only patients with a history of more of one year of follow-up demonstrated significant associations among -1082A allele and disease recurrence (defined as 2 or more episodes) toxoplasmic retinochoroiditis ( p = 0.03; x² = 4,602).

Conclusions:: Our data suggest that the polymorphism in the locus -1082 of IL-10 gene may not be associated with occurrence of toxoplasmic retinochoroiditis, but may be associated with the recurrence of the disease in humans.

Keywords: immunomodulation/immunoregulation • chorioretinitis • gene/expression 
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