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M. V. Volin, G. L. Searle, M. D. Silverman; Chemokine Expression by Cytokine-stimulated Human Iris Stromal Cells. Invest. Ophthalmol. Vis. Sci. 2007;48(13):5193.
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Acute anterior uveitis is an inflammatory disorder of the iris and ciliary body. To better understand the role of resident cells in the progression of acute anterior uveitis, human iris stromal cells were isolated and their production of chemokines was assessed in vitro.
Iris stromal cells were isolated from anonymously donated human eyes. Iris stromal cells in culture were stimulated with TNFα (10ng/ml) and IFNγ 10ng/ml) for 3 or 24 hours. Chemokine production by human iris stromal cells was determined using human chemokine antibody arrays capable of detecting 38 different chemokines. Chemokine production by human iris stromal cells was quantified using specific ELISA assays and Western blotting.
Antibody array experiments identified the increased production of several chemokines by cytokine-stimulated iris stromal cells (n=2). The most dramatic increases were seen in production of IL-8, IP-10, RANTES, and GRO. Western blotting using anti-RANTES or anti-IP-10 confirmed increased production of RANTES or IP-10 respectively by iris stromal cells following 3 or 24 hours of cytokine stimulation. Specific RANTES ELISA showed that TNFα and IFNγ-stimulation of iris stromal cells significantly increased their release of RANTES into the culture media (n=3, p<0.05). Specific IP-10 ELISA showed that TNFα and IFNγ-stimulation of iris stromal cells increased their release of IP-10 into the culture media (n=3, p<0.05).
Cytokines such as TNFα and IFNγ are known to play an important role in the development of uveitis. Here we show that these cytokines can induce resident iris stromal cells to produce chemokines IL-8, IP-10, RANTES, and GRO in vitro. These results indicate that iris stromal cells can play a part in the recruitment of leukocytes into the anterior segment of the eye in anterior uveitis.
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