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T. Hattori, T. Kezuka, Y. Usui, M. Takeuchi, Y. Okunuki, M. Kurita, R. Yamashita, M. Haneda, S. Shirato, M. Usui; Human Iris Pigment Epithelial (hIPE) Cells Possess a Capacity to Modulate T Cell Activation. Invest. Ophthalmol. Vis. Sci. 2007;48(13):5194.
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To determine whether Human iris pigment epithelial (hIPE) cells have a capacity to suppress T cell activation. And if so, mechanism of suppression capacity was revealed.
Human iris pigment epithelial (hIPE) cells were prepared from Glaucoma patients undertaken surgery, and were incubated in vitro 4-7 days in RPMI1640 medium containing 10% FCS. Expression of MHC molecules and costimulatory molecules on cultured hIPE cells stimulated with or without IFN-g were examined by FACS. In addition, periphral T cells were incubated with cultured hIPE cells prepared from the same patients and anti-CD3 antibody in transwell culture system, or in the presence of anti-B7-H1 and B7-DC antibodies, and T cell proliferation was assessed by [3H]-thymidine incorporation.
Cultured hIPE cells expressed MHC Class I, B7-H1, and B7-DC, and these expression were enhanced and MHC Class II was newly expressed by stimulation with IFN-g. Anti-CD3-driven T cell proliferation was inhibited by cultured hIPE cells, which inhibition was not observed in transwell culture system, or in the presence of anti-B7-H1 and B7-DC antibodies.
Cultured hIPE cells prevent T cells proliferation via cell-to-cell contact, in which B7-H1 and B7-DC pathways are related.
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