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M. Matsubara, S. Takeda, K. Mukai, Y. Ishii; PMMA, Medium- or High-Dk RGP Orthokeratology Extended Wear; Histological Outcomes in Rabbit Corneas After One Week Extended Wear. Invest. Ophthalmol. Vis. Sci. 2007;48(13):5392.
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© ARVO (1962-2015); The Authors (2016-present)
To determine the relationship between hypoxia and redistribution of epithelial cells with different Dk orthokeratology lens (OK lens) wear by comparing the histological effect on the rabbit corneal epithelium.
Rabbits (n=18) were fitted with either a Dk 0 ( PMMA) , 33 or 95 OK lens and sacrificed after one week of continuous wear. HE, PAS and enzyme staining were performed on corneal tissues obtained from them.
Redistribution of epithelial thickness was found in every cornea regardless of Dk values. At the area corresponding to the alignment curve zone of OK lens, the epithelium was thick with increased layers as many as 10 to 12. Epithelial edema was not observed. Central epithelium was very thin or detached in Dk 0 group. PAS staining showed decreased glycogranules in basal cells in Dk 0 group whereas no change was found in other groups. Lactic dehydrogenase and alkaline phosphatase showed slightly strong stainings, but there was no difference among the groups.
No conspicuous histological changes after adapted 1 week continuous wear of OK lens with Dk 33 or 95 suggest the good potential of epithelial adaptation to orthokeratology.
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