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C. A. Kramann, N. Wiegel, N. Pfeiffer, F. H. Grus; Effects of Contact Lenses on the Protein Composition in Tear Film: A Proteinchip Study. Invest. Ophthalmol. Vis. Sci. 2007;48(13):5408.
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The aim of this study was to analyze the effects of hard and soft contact lenses on the protein composition of tears in contact lens wearers.
Wearers of soft contact lenses (n=13) and hard contact lenses (n=13) were recruited. 13 non-contact lens wearers were also included as control. Tears were collected using Schirmer strips with anesthesia and frozen until use. The tears were eluted and analyzed on ProteinChips Seldi-TOF (Surface-enhanced laser-desorption and ionization in time of flight mass spectrometry; BioRad, USA) with different chromatographic surfaces (cationic and anionic exchanger, and reversed phase surface). The Seldi spectra were analyzed by multivariate statistical analysis and artificial neural networks in order to find a biomarker panel which differentiate best between the groups. Some of these protein biomarkers were identified using Maldi-TOFTOF.
The tear protein composition differed significantly from protein composition in non-contact lens wearers in both wearers of hard and soft contact lenses (p<0.01). However, the deviation of tear protein patterns were more pronounced in wearers of soft contact lenses. Nevertheless, considering typical dry-eye biomarkers found in previous studies (Grus et al., IOVS 2005), e.g. the PRP4 (proline rich protein) differed in both soft and hard contact lens wearers in comparison to the non-contact lens wearers.
Contact lens wear alters the protein profiles in a complex manner. This study could demonstrate that those changes can not only be found in wearers of soft contact lenses, but also in hard contact lens wearers.
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