May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Human Trans-Scleral Albumin Diffusion and the Effect of Topographical Location and Donor Age
Author Affiliations & Notes
  • O. A. Anderson
    The Rayne Institute, St Thomas' Hospital, London, United Kingdom
  • T. L. Jackson
    The Rayne Institute, St Thomas' Hospital, London, United Kingdom
  • J. K. Singh
    The Rayne Institute, St Thomas' Hospital, London, United Kingdom
  • A. A. Hussain
    The Rayne Institute, St Thomas' Hospital, London, United Kingdom
  • J. Marshall
    The Rayne Institute, St Thomas' Hospital, London, United Kingdom
  • Footnotes
    Commercial Relationships O.A. Anderson, None; T.L. Jackson, None; J.K. Singh, None; A.A. Hussain, None; J. Marshall, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 5831. doi:
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      O. A. Anderson, T. L. Jackson, J. K. Singh, A. A. Hussain, J. Marshall; Human Trans-Scleral Albumin Diffusion and the Effect of Topographical Location and Donor Age. Invest. Ophthalmol. Vis. Sci. 2007;48(13):5831.

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Abstract

Purpose:: To quantify the diffusion of albumin across human sclera and to assess topographical and age-related variation.

Methods:: Equatorial superotemporal scleral tissue from 15 donor eyes (mean age 60 years, range 39-84 years) were mounted in a modified Ussing chamber. Additional tissue was taken from the anterior and posterior superotemporal regions of 6 eyes, and equatorial superonasal, and inferotemporal regions of a further 6 eyes. Fluorescein isothiocyanate (FITC) labeled, 0.412 mMol, bovine albumin was placed in one hemi-chamber facing the internal scleral surface, and the rate of trans-scleral diffusion was determined over 24 hours, at 25oC, with a spectrophotometer.

Results:: Mean diffusion for equatorial superotemporal scleral tissue +/- 1SD was 12.35 +/- 7.12 pmoles/hour/mm2. There was no significant difference in diffusion over the different topographical regions tested. The effect of donor age was assessed for the fifteen equatorial superotemporal samples. Regression analysis showed a significant decline in scleral diffusion of albumin with increasing donor age (p=0.0166).

Conclusions:: The study of normal scleral physiology may guide strategies for trans-scleral drug delivery and help understand disease states characterised by abnormal trans-scleral diffusion. The above results quantify trans-scleral diffusion of albumin and assesses its relationship with topographical location and donor age. The decrease in albumin permeability with increasing donor age may have pharmacokinetic implications.

Keywords: sclera • aging • anatomy 
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