May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Induction of ßIg-h3 Protein by Tgfß1 in Corneal Epithelial Cells and Fibroblasts
Author Affiliations & Notes
  • T.-I. Kim
    Ophthalmology, Vision research institute, Seoul, Republic of Korea
  • Y. Byoun
    Ophthalmology, Vision research institute, Seoul, Republic of Korea
  • S. Choi
    Ophthalmology, Vision research institute, Seoul, Republic of Korea
  • S. Kim
    Ophthalmology, Vision research institute, Seoul, Republic of Korea
  • J. Park
    Daegu Yonsei eye clinic, Daegu, Republic of Korea
  • H. Lee
    Ophthalmology, Vision research institute, Seoul, Republic of Korea
  • E. Kim
    Ophthalmology, Vision research institute, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships T. Kim, None; Y. Byoun, None; S. Choi, None; S. Kim, None; J. Park, None; H. Lee, None; E. Kim, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 5865. doi:
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      T.-I. Kim, Y. Byoun, S. Choi, S. Kim, J. Park, H. Lee, E. Kim; Induction of ßIg-h3 Protein by Tgfß1 in Corneal Epithelial Cells and Fibroblasts. Invest. Ophthalmol. Vis. Sci. 2007;48(13):5865.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: To compare the production of ßig-h3 protein and its induction by TGFß1 in normal corneal epithelial cells and cultured corneal fibroblasts of normal and granular corneal dystrophy (GCD) type II heterozygote and homozygote.

Methods:: 0, 10, and 20ng/ml of TGFß1 were treated in corneal epithelial cells and 5th-6th generation corneal fibroblasts of both normal and GCD type II heterozygote and homozygote in FBS(fetal bovine serum) free culture media, then western blot and RT-PCR were followed to measure the level of ßig-h3 protein.

Results:: Increased production of ßig-h3 protein was observed in both corneal epithelial cells and fibroblasts of all three groups after 24 hours of incubation and it was directly related to the concentration of and time treated with TGFß1. Level of mRNA transcription of ßig-h3 protein was comparable in both corneal epithelial and fibroblasts and its production was increased after treatment with TGFß1.

Conclusions:: Both corneal epithelial cells and fibroblasts of normal and GCD type II homozygotes and heterozygotes produced ßig-h3 protein, and TGFß1 was a inducing factor of ßig-h3 protein production.

Keywords: cornea: stroma and keratocytes • cornea: epithelium • protein structure/function 
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