May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Histological Evaluation of Mechanical Epithelial Separation Using Epi–LASIK Procedure
T. Soma; M. Yamato; S. Kosaka; N. Maeda; T. Okano; Y. Tano; K. Nishida
Author Affiliations & Notes
  • T. Soma
    Department of Ophthalmology, Osaka University Medical School, Suita, Japan
  • M. Yamato
    Institute of Advanced Biomedical Engineering and Science,
    Tokyo Women's Medical University, Tokyo, Japan
  • S. Kosaka
    Medical Research Institute,
    Tokyo Women's Medical University, Tokyo, Japan
  • N. Maeda
    Department of Ophthalmology, Osaka University Medical School, Suita, Japan
  • T. Okano
    Institute of Advanced Biomedical Engineering and Science,
    Tokyo Women's Medical University, Tokyo, Japan
  • Y. Tano
    Department of Ophthalmology, Osaka University Medical School, Suita, Japan
  • K. Nishida
    Department of Ophthalmology, Tohoku University School of Medicine, Sendai, Japan
  • Footnotes
    Commercial Relationships  T. Soma, None; M. Yamato, None; S. Kosaka, None; N. Maeda, None; T. Okano, None; Y. Tano, None; K. Nishida, None.
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 517. doi:
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      T. Soma, M. Yamato, S. Kosaka, N. Maeda, T. Okano, Y. Tano, K. Nishida; Histological Evaluation of Mechanical Epithelial Separation Using Epi–LASIK Procedure . Invest. Ophthalmol. Vis. Sci. 2006;47(13):517.

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Abstract

Purpose: : To evaluate the effect of mechanical epithelial separation with an epikeratome on the histological ultrastructure of epithelial flaps and stromal beds from human corneas.

Methods: : Four USA–eye–bank eyes were de–epithelialized using an epikeratome to mechanically separate the epithelial layer. Epithelial flaps and stromal beds were assessed by light microscopy, and transmission and scanning electron microscopy. We also examined immunofluorescence staining for type IV and type VII collagens which compose the lamina densa and the anchoring fibril, respectively.

Results: : Electron microscopy demonstrated that the epithelial layer was separated partially at the level of the lamina reticularis and partially at the level of the lamina lucida. Immunofluorescence study showed that the positive staining of type VII collagen and the discontinuous staining of type IV collagen were observed in the stromal bed specimens, and the discontinuous staining of type VI and VII collagens was observed in the epithelial flap specimens.

Conclusions: : The epithelial flap is mechanically separated using epi–LASIK procedure partially at the level of the lamina reticularis and partially at the level of the lamina lucida.

Keywords: refractive surgery • microscopy: electron microscopy • immunohistochemistry 
© 2006, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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