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T. Soma, M. Yamato, S. Kosaka, N. Maeda, T. Okano, Y. Tano, K. Nishida; Histological Evaluation of Mechanical Epithelial Separation Using Epi–LASIK Procedure . Invest. Ophthalmol. Vis. Sci. 2006;47(13):517.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the effect of mechanical epithelial separation with an epikeratome on the histological ultrastructure of epithelial flaps and stromal beds from human corneas.
Four USA–eye–bank eyes were de–epithelialized using an epikeratome to mechanically separate the epithelial layer. Epithelial flaps and stromal beds were assessed by light microscopy, and transmission and scanning electron microscopy. We also examined immunofluorescence staining for type IV and type VII collagens which compose the lamina densa and the anchoring fibril, respectively.
Electron microscopy demonstrated that the epithelial layer was separated partially at the level of the lamina reticularis and partially at the level of the lamina lucida. Immunofluorescence study showed that the positive staining of type VII collagen and the discontinuous staining of type IV collagen were observed in the stromal bed specimens, and the discontinuous staining of type VI and VII collagens was observed in the epithelial flap specimens.
The epithelial flap is mechanically separated using epi–LASIK procedure partially at the level of the lamina reticularis and partially at the level of the lamina lucida.
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