May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Stat3–Activation Negatively Correlates With Rhodopsin–Expression in Adult Retina with Inflammation
Author Affiliations & Notes
  • Y. Ozawa
    Keio Univ. School of Medicine, Tokyo, Japan
    Laboratory of Retinal Cell Biology, Department of Ophthalmology,
  • T. Kurihara
    Keio Univ. School of Medicine, Tokyo, Japan
    , Laboratory of Retinal Cell Biology, Department of Ophthalmology,
  • N. Nagai
    Keio Univ. School of Medicine, Tokyo, Japan
    Laboratory of Retinal Cell Biology, Department of Ophthalmology,
  • T. Koto
    Keio Univ. School of Medicine, Tokyo, Japan
    Laboratory of Retinal Cell Biology, Department of Ophthalmology,
  • S. Satofuka
    Keio Univ. School of Medicine, Tokyo, Japan
    Laboratory of Retinal Cell Biology, Department of Ophthalmology,
  • M. Inoue
    Keio Univ. School of Medicine, Tokyo, Japan
    Department of Ophthalmology,
  • Y. Oike
    Keio Univ. School of Medicine, Tokyo, Japan
    Laboratory of Retinal Cell Biology,
  • K. Tsubota
    Keio Univ. School of Medicine, Tokyo, Japan
    Department of Ophthalmology,
  • S. Ishida
    Keio Univ. School of Medicine, Tokyo, Japan
    Laboratory of Retinal Cell Biology, Department of Ophthalmology,
  • H. Okano
    Keio Univ. School of Medicine, Tokyo, Japan
    Department of Physiology,
    Core Research for Evolutional Science and Technology (CREST), Tokyo, Japan
  • Footnotes
    Commercial Relationships  Y. Ozawa, None; T. Kurihara, None; N. Nagai, None; T. Koto, None; S. Satofuka, None; M. Inoue, None; Y. Oike, None; K. Tsubota, None; S. Ishida, None; H. Okano, None.
  • Footnotes
    Support  the Ministry of Education, Science, and Culture of Japan (MEXT) and a grant–in–aid from the 21st Century COE program
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 1065. doi:
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      Y. Ozawa, T. Kurihara, N. Nagai, T. Koto, S. Satofuka, M. Inoue, Y. Oike, K. Tsubota, S. Ishida, H. Okano; Stat3–Activation Negatively Correlates With Rhodopsin–Expression in Adult Retina with Inflammation . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1065.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : We have previously reported that STAT3–activation negatively controls Rhodopsin–expression in perinatal retina during developmental processes (Ozawa et al. Molecular and Cellular Neuroscience 2004). Next interest is whether this regulation is also available in adult. In this study, we evaluate the level of STAT3–activation and Rhodopsin–expression during severe inflammation in adult retina.

Methods: : Lipopolysaccharide (LPS) was injected into adult C57/B6 mice intrapelitoneally to establish the model of retinal inflammation (a model of endotoxin–induced uveitis) in which IL–6 is known to be upregulated. Levels of STAT3–activation and Rhodopsin–expression were analyzed in each time point after LPS injection. Dark–adapted full–field electroretinography (ERG) was recorded at 24 hours after LPS injection.

Results: : STAT3–activation was upregulated from 3 hours after intraperitoneal injection of LPS, peaking at 8 hours and decreased but still continued at 48 hours. Correlated to this, Rhodopsin–expression started to be downregulated at 16 hours and continuously decreased. Dark–adapted full–field electroretinography (ERG) clearly showed reduction in amplitude of a–wave which represents function of photoreceptor cells.

Conclusions: : Strong STAT3–activation inhibited Rhodopsin–expression in adult retina with inflammation. Although the mechanism of negative effect of STAT3–activation on Rhodopsin–expression could be regulated by multiple stimuli at inflammation, excess STAT3–activation negatively correlated with maintainance of Rhodopsin–expression thus showed abnormality in ERG.

Keywords: inflammation • photoreceptors • retina 
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