May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Clathrin and Adaptin in Drusen, Bruch's Membrane and Choroid in AMD and Non–AMD Donor Eyes
H. Bando; K.G. Shadrach; M.E. Rayborn; J.W. Crabb; J.G. Hollyfield
Author Affiliations & Notes
  • H. Bando
    Cole Eye Institute, The Cleveland Clinic Foundation, Cleveland, OH
  • K.G. Shadrach
    Cole Eye Institute, The Cleveland Clinic Foundation, Cleveland, OH
  • M.E. Rayborn
    Cole Eye Institute, The Cleveland Clinic Foundation, Cleveland, OH
  • J.W. Crabb
    Cole Eye Institute, The Cleveland Clinic Foundation, Cleveland, OH
  • J.G. Hollyfield
    Cole Eye Institute, The Cleveland Clinic Foundation, Cleveland, OH
  • Footnotes
    Commercial Relationships  H. Bando, None; K.G. Shadrach, None; M.E. Rayborn, None; J.W. Crabb, None; J.G. Hollyfield, None.
  • Footnotes
    Support  NIH Grant EY014239, EY014240, EY015638
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 859. doi:
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      H. Bando, K.G. Shadrach, M.E. Rayborn, J.W. Crabb, J.G. Hollyfield; Clathrin and Adaptin in Drusen, Bruch's Membrane and Choroid in AMD and Non–AMD Donor Eyes . Invest. Ophthalmol. Vis. Sci. 2006;47(13):859.

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Abstract

Purpose: : Clathrins were identified in our recent proteomic analysis of drusen from age–related macular degeneration (AMD) donor eyes. This study was conducted to define the localization of clathrins in drusen, Bruch’s membrane and choroid of AMD tissues and to compare this distribution with that in non–AMD control tissues. Additionally, we investigated the distribution of adaptin, which is closely linked to the function of clathrin.

Methods: : Human eyes were from donors between 66 and 94 years of age; 10 eyes were from donors with AMD and 10 from non–AMD donors. Bruch’s membrane and choroid from the macula from each donor eye were prepared for immunohistochemistry and Western blotting and differences in immunoreactivity were quantitated.

Results: : Drusen, Bruch’s membrane and choroid from AMD tissues showed greater immunoreactivity for clathrin and adaptin than did non–AMD tissues. Western blots also showed more intense clathrin and adaptin signals from AMD than were present in non–AMD tissues.

Conclusions: : Clathrin and adaptin appear to be more abundant in drusen, Bruch’s membrane, choroid of AMD compared to non–AMD controls. This study suggests that accumulation of clathrin and adaptin in drusen, Bruch’s membrane and choroid may reflect a higher rate of clathrin mediated endocytosis in AMD tissues. Alternatively, the accumulation of these proteins may reflect a higher susceptibility to oxidative damage.

Keywords: age-related macular degeneration • drusen • oxidation/oxidative or free radical damage 
© 2006, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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