May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Hydrostatic Pressure Induces Mitochondrial Fission in Differentiated RGC–5 Cells
Author Affiliations & Notes
  • W.–K. Ju
    Ophthalmology, Hamilton Glaucoma Center, La Jolla, CA
  • Q. Liu
    Ophthalmology, Hamilton Glaucoma Center, La Jolla, CA
  • J. Lindsey
    Ophthalmology, Hamilton Glaucoma Center, La Jolla, CA
  • R. Weinreb
    Ophthalmology, Hamilton Glaucoma Center, La Jolla, CA
  • J. Crowston
    Ophthalmology, Hamilton Glaucoma Center, La Jolla, CA
  • Footnotes
    Commercial Relationships  W. Ju, None; Q. Liu, None; J. Lindsey, None; R. Weinreb, None; J. Crowston, None.
  • Footnotes
    Support  Vision for Glaucoma (La Jolla); NIH/NEI Grant EY014661 (JDL)
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 1253. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      W.–K. Ju, Q. Liu, J. Lindsey, R. Weinreb, J. Crowston; Hydrostatic Pressure Induces Mitochondrial Fission in Differentiated RGC–5 Cells . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1253.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Mitochondrial fission is a cellular response to stress and may play an important role in neuronal apoptosis in neurodegenerative disease. It is not known whether increased intraocular pressure, a risk factor for glaucoma, induces mitochondrial fission in retinal ganglion cells (RGC). The purpose of this study was to determine whether increased hydrostatic pressure induces mitochondrial fission in cultured retinal ganglion cells.

Methods: : RGC–5 cells were differentiated with succinyl concanavalin A (50 µg/ml). Using a modified pressure chamber, 30 mmHg of pressure was applied to differentiated RGC–5 cells for 1, 2, and 3 days. RGC–5 cells were then labeled with MitoTracker Red and mitochondrial morphology was assessed by fluorescence microscopy.

Results: : RGC–5 cells did not show mitochondrial fission up to 2 days of pressure. However, 3 days of pressure resulted in more than 80% of mitochondrial fission characterized by the conversion of tubular fused mitochondria into isolated small organelles. Non–pressurized control cells showed only 15% of mitochondrial fission at 3 days.

Conclusions: : Elevated pressure induces mitochondrial fission in differentiated RGC–5 cells. Mitochondrial fission may provide a biomarker for RGC stress and may play an important role in pressure–induced RGC death.

Keywords: mitochondria • ganglion cells • intraocular pressure 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×