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G. Aguilar, C. Santacruz, M. Linares, J. Rivera, L. López, J. López–López, R. Suárez, J. Zenteno, Y. Garfias, M. Jimenez–Martínez; In vitro Allergen Stimulation Induces CD30 Expression and Cytokine Production on T Cells From Patients With Allergic Conjunctivitis . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1288.
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Allergic conjunctivitis (AC) is related to Th2 immune responses. Recently it has been suggested that CD4+CD30+ T cells are the main subset that proliferate in response to allergen stimulation. CD30 is an activation molecule widely distributed on leukocytes.
The aim of this work was to evaluate the expression of CD30 on T cells subsets and cytokine production after in vitro stimulation in patients with AC.
Patients were divided into two groups: Group A were patients with the clinical diagnosis (CD)of AC and skin prick test (SPT)positive to Dermatophagoides pteronyssinus (Der p) (n=4). Group B were patients with CD of AC and SPT negative to any allergen (n=4). Peripheral blood mononuclear cells were cultured and stimulated with Der p for 7 days, after that, cells were labelled with fluorescent Abs against CD30, CD4, CD8, IFN–g and IL–4. Results were analyzed by flow–cytometry and rank sum test was used for statistical analysis.
Percentage of CD4+CD30+ T cells in group A was 31% ± 8 vs 35% ± 10 in group B; percentage of CD8+CD30+ T cells in group A was 38% ± 7 vs 31 ± 11 in group B; Intracellular expression (IE) of IFN–g on CD4+CD30+ T cells in group A was 27% ± 4 vs IE of IFN–g on CD4+CD30– T cells in same group 11% ± 2 (p=0.024); IE of IL–4 on CD4+CD30+ T cells in group A was 27% ± 3 vs IE of IL–4 on CD4+CD30– T cells in same group 6% ± 2(p=0.024); IE of IFN–g on CD4+CD30+ T cells in group B was 26% ± 8 vs IE of IFN–g on CD4+CD30– T cells in same group 11% ± 3 (p=0.042); IE of IL–4 on CD4+CD30+ T cells in group B was 22% ± 7 vs IE of IL–4 on CD4+CD30– T cells in same group 9% ± 3(p=0.073); IE of IFN–g on CD8+CD30+ T cells in group A was 17% ± 4 vs IE of IFN–g on CD8+CD30– T cells in same group 8% ± 2 (p=0.012);IE of IL–4 on CD8+CD30+ T cells in group A was 16% ± 6 vs IE of IL–4 on CD8+CD30– T cells in same group 4% ± 1(p=0.019); IE of IL–4 on CD8+CD30+ T cells in group B was 11% ± 3 vs IE of IL–4 on CD8+CD30– T cells in the same group 3% ± 2(p=0.006).
Our results showed that CD30+ T cells (CD4 and CD8) were the main cell subset producer of cytokines; it is the first demonstration that cytotoxic T cells bearing CD30 produce cytokines after allergenic stimulation in patients with AC. Our results suggest that CD30+ T cells could regulate the immune response toward Th2 profile associated to development of ocular allergy.
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