May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Corneal Antigen–Presenting Cell Depletion After Prolonged Storage of Corneal Buttons
Author Affiliations & Notes
  • X. Zhang
    The Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA
  • Y. Jin
    The Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA
  • L. Chen
    The Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA
  • L. shen
    The Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA
  • S. Rashid
    The Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA
  • Q. Zhang
    The Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA
  • R. Dana
    The Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA
  • Footnotes
    Commercial Relationships  X. Zhang, None; Y. Jin, None; L. Chen, None; L. shen, None; S. Rashid, None; Q. Zhang, None; R. Dana, None.
  • Footnotes
    Support  NIH Grant EY–R01–12963
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 1297. doi:
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      X. Zhang, Y. Jin, L. Chen, L. shen, S. Rashid, Q. Zhang, R. Dana; Corneal Antigen–Presenting Cell Depletion After Prolonged Storage of Corneal Buttons . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1297.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Prolonged storage (7–14 days) of donor cornea has been shown to prevent allograft rejection in both human and mouse studies, especially in high risk corneal transplantation for reasons that have remained obscure. We hypothesize that the mechanism is associated with corneal antigen–presenting cell (APC) depletion from the corneal donor tissue, thereby diminishing direct–type allo–sensitization by graft–derived APCs.

Methods: : Donor corneas harvested from C57Bl/6 mice were either stored in Optisol–GS® medium at 4 ºC for 14 days or used fresh as a control group. To determine the number of APCs in the corneas, both groups were immunohistochemically stained with the pan–leukocyte surface marker CD45. CD45 positive cells were then enumerated on whole–mount corneas using a confocal microscope.

Results: : The CD45 positive cell density was significantly decreased in the corneas that were stored for 14 days compared with the fresh corneas.

Conclusions: : Prolonged storage of corneal tissue reduces the number of APCs in the cornea, and may be the underlying reason for increased allograft survival rates using these corneal buttons stored for prolonged periods as cornea grafts.

Keywords: antigen presentation/processing • cornea: storage • immunohistochemistry 
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