May 2006
Volume 47, Issue 13
ARVO Annual Meeting Abstract  |   May 2006
Blue–Light Irradiation Induces Oxidation of Phospholipids in Mouse Retina
Author Affiliations & Notes
  • M. Suzuki
    Ophthalmology, Osaka University, Suita, Japan
  • M. Kamei
    Ophthalmology, Osaka University, Suita, Japan
  • H. Itabe
    Department of Biological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan
  • Y. Tano
    Ophthalmology, Osaka University, Suita, Japan
  • Footnotes
    Commercial Relationships  M. Suzuki, None; M. Kamei, None; H. Itabe, None; Y. Tano, None.
  • Footnotes
    Support  Grant–in–Aid for Scientific Research (#15591853) from the Ministry of Education, Science and Culture of Japan
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 1390. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      M. Suzuki, M. Kamei, H. Itabe, Y. Tano; Blue–Light Irradiation Induces Oxidation of Phospholipids in Mouse Retina . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1390.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose: : Oxidative stress is suggested to be one of the key factors in the pathogenesis of age–related macular degeneration (AMD). We previously showed that oxidized phospholipids increased in AMD eyes compared with age–matched normal eyes. To establish an AMD animal model, we attempted to induce the oxidation of phospholipids in mouse retina by irradiating blue–light.

Methods: : BALB/c mice were exposed to a blue–light–emitting diode (illuminance: 5 mW/cm2, transmission peak wavelength: 480 nm) continuously for 30 days in a specially designed shadow–free cage. To detect oxidized phospholipids, immunohistochemistry with the anti–oxidized phosphatidylcholine antibody, DLH3, was performed on cryosections of the eye. Oxidized phospholipids were measured by competitive ELISA. Mice caged under normal light served as a control.

Results: : Positive staining for oxidized phospholipids was observed in the outer nuclear layer (ONL) and retinal pigment epithelium (RPE) of the blue–light–exposed eyes, while control eyes showed minimal immunoreactivity. Long term blue–light exposure caused thinning of the ONL and photoreceptor layer. Competitive ELISA showed that blue–light exposure induced a 2.0–fold increase in oxidized phospholipids compared with control.

Conclusions: : These results indicate that long term blue–light irradiation induces the oxidation of phospholipids in the ONL and RPE of mice. Blue–light may be useful for developing an animal model of AMD.

Keywords: age-related macular degeneration • oxidation/oxidative or free radical damage • lipids 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.