May 2006
Volume 47, Issue 13
ARVO Annual Meeting Abstract  |   May 2006
Toxicity of Methylprednisolone on ARPE–19 and R28 Cells
Author Affiliations & Notes
  • A. Neekhra
    Ophthalmology, University of California Irvine, Irvine, CA
  • R. Narayanan
    Ophthalmology, University of California Irvine, Irvine, CA
  • S. Luthra
    Ophthalmology, University of California Irvine, Irvine, CA
  • G. Seigel
    Ophthalmology, Ross Eye Institute,University at Buffalo, Buffalo, NY
  • M.C. Kenney
    Ophthalmology, University of California Irvine, Irvine, CA
  • B.D. Kuppermann
    Ophthalmology, University of California Irvine, Irvine, CA
  • Footnotes
    Commercial Relationships  A. Neekhra, None; R. Narayanan, None; S. Luthra, None; G. Seigel, None; M.C. Kenney, None; B.D. Kuppermann, None.
  • Footnotes
    Support  Discovery Eye Foundation, Iris and B. Gerald Cantor Foundation, Research to Prevent Blindness Foundation
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 1404. doi:
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      A. Neekhra, R. Narayanan, S. Luthra, G. Seigel, M.C. Kenney, B.D. Kuppermann; Toxicity of Methylprednisolone on ARPE–19 and R28 Cells . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1404.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To study the in vitro toxicity of methylprednisolone on ARPE–19 and R28 cell lines.

Methods: : ARPE–19 and R28 cells were grown to 100% confluency in serum free DMEM media. Methyl prednisolone acetate suspension (MPA) (Pharmacia & Upjohn Company, Kalamazoo, MI ) was centrifuged to obtain drug (pellet) and supernatant fluid (vehicle).The cells were treated with four different concentrations of drug: 25,50,100 and 500 µg/ml and corresponding four concentrations of vehicle: 18.75, 37.5, 75 and 375 µg/ml. Cell viability was measured at 2, 6 and 24 hours using the trypan blue dye exclusion assay.

Results: : The mean cell viabilities of ARPE–19 cells after 24 hours exposure to 25,50,100 and 500 µg/ml of pellet were 93.7 ± 1.9% (p>0.05), 84.1±1.2%(p<0.001), 59.4 ±2.6%(p<0.001) and 5.6± 2.0% (p<0.001) respectively, compared to untreated cells that had a viability of 94.7±1.3 % . Cells treated with corresponding concentrations of preservative alone (18.75, 37.5, 75 and 375 µg/ml) had viabilities of 94.2±2.3%, 95.3±2.0%, 94.5±2.1% and 94.6 ±1.6% respectively, which were not significantly different from the viability of untreated ARPE–19 cells. Similarly, the mean cell viabilities of R28 cells after exposure to MPA pellet (25,50,100 and 500 µg/ml) were 90.4± 0.5%(p>0.05),85.7±1.8%(p<0.01), 55.3± 4.2%(p<0.001) and 9.3±1.0% (p<0.001) respectively, compared to untreated R28 cells that had a mean viability of 91.0± 1.0% while the highest corresponding concentration of preservative of 375 µg/ml was not toxic with cell viability of 89.5 ±0.75%.

Conclusions: : MPA is toxic to ARPE–19 and R28 cells in vitro at the clinical intravitreal dose of 100µg/ml while the preservative is not toxic. This toxicity profile of MPA is similar to that of triamcinolone acetonide in vitro, making it a less valuable alternative for intravitreal injections or implants

Keywords: retinal culture • corticosteroids • injection 

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