May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
P27, P63, Cd95, Bax, Bcl–2, Cathepsin and Survivin Gene Expression in Corneas of Patients With Fuchs’ Dystrophy
Author Affiliations & Notes
  • N. Szentmary
    Department of Ophthalmology, Semmelweis University, Budapest, Hungary
  • B. Szende
    Department of Ophthalmology, Semmelweis University, Budapest, Hungary
  • I. Süveges
    Department of Ophthalmology, Semmelweis University, Budapest, Hungary
  • Footnotes
    Commercial Relationships  N. Szentmary, None; B. Szende, None; I. Süveges, None.
  • Footnotes
    Support  OTKA T048477
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 1594. doi:
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      N. Szentmary, B. Szende, I. Süveges; P27, P63, Cd95, Bax, Bcl–2, Cathepsin and Survivin Gene Expression in Corneas of Patients With Fuchs’ Dystrophy . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1594.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To elucidate the pathways of genetically programmed cell death (apoptosis) of corneas with Fuchs' dystrophy.

Methods: : Sixteen corneal buttons (14 patients, 42.8% males, age 72.3 ± 11.3 years) with Fuchs' dystrophy and 4 buttons (4 patients, 50% males, age 67.2 ± 15.9 years) from enucleated eyes with chorioideal melanoma (controls) were analysed histologically. Immunohistochemical analysis was performed to investigate the expression of p63, p27, survivin, CD95, cathepsin, bax and bcl–2.

Results: : P63 (62.5 / 100%) and P27 (100% both) were positive, bcl–2 (0% both) and bax (6.6 / 0%) were negative in both Fuchs' dystrophy and control group corneas. Survivin (46.1 / 0%) and CD95 (57.1 / 33.3%) positivity was detected more in Fuchs’dystrophy than in normal corneas. Cathepsin was expressed more frequently in Fuchs’ dystrophy corneas (90.9%) compared to controls (75%), however with weaker immunohistochemical reaction.

Conclusions: : The genetically programmed cell death is CD95 dependent and not related to the P53 and cathepsin way in Fuchs' dystrophy corneas. Detailed regulatory mechanisms remain to be clarified.

Keywords: cornea: basic science • apoptosis/cell death • cornea: epithelium 
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