Abstract
Purpose: :
To identify differentiate genes concerned with apoptosis that are up–regulated or down–regulated in OLETF corneal keratocyte stimulated interleukin–1α.
Methods: :
Primary cultures of OLETF (Otsuka Long Evans Tokushima Fatty, Japan) corneal keratocyte were cultured, and treated with 20 ng/ml of IL–1a for 8 hours. Hybridization was carried out using Oligonucleotide microarray. A normal keratocyte sample was labeled with Cy3, and diabetic keratocytes sample was labeled with Cy5. The confirmation of gene was performed by RT–PCR.
Results: :
Diabetes showed down–regulated 20 genes and up–regulated 24 genes compared with normal keratocyte. Also IL–1α treated diabetic keratocytes expressed 31 down–regulated and 33 up–regulated genes. Compared with diabetic keratocytes, the new expression of genes in OLETF treated with IL–1α were as BCL–1 oncogene, suppressor of cytokine signaling, lamimin, chemokine ligand, tissue inhibitor of metalloproteinase, matrix metalloproteinase, bone morphogenetic protein, Lipocalin 2, complement component.
Conclusions: :
There were differentially expressed genes related with apoptosis between normal and diabetic keratocytes of OLETF. Also IL–1α may stimulate to differing effects of apoptosis on diabetic corneal keratocytes. Studies to analyze the apoptotic significance of the identified differences of diabetes are needed.
Keywords: gene/expression • apoptosis/cell death • diabetes