May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Normal Rat Retina Exhibits A Higher Protein Synthesis Rate Than Skeletal Muscle
P.E. Fort; K.A. Gilbert; C.L. Bradley; R.S. J. Singh; C.E. N. Reiter; L.S. Jefferson; S.R. Kimball; T.W. Gardner; Penn State Retina Research Group
Author Affiliations & Notes
  • P.E. Fort
    Penn State College of Medicine, Milton S. Hershey Medical Center, HERSHEY, PA
    Department of Ophthalmology,
  • K.A. Gilbert
    Penn State College of Medicine, Milton S. Hershey Medical Center, HERSHEY, PA
    Department of Ophthalmology,
  • C.L. Bradley
    Penn State College of Medicine, Milton S. Hershey Medical Center, HERSHEY, PA
    Department of Physiology,
  • R.S. J. Singh
    Penn State College of Medicine, Milton S. Hershey Medical Center, HERSHEY, PA
    Department of Ophthalmology,
  • C.E. N. Reiter
    Penn State College of Medicine, Milton S. Hershey Medical Center, HERSHEY, PA
    Department of Ophthalmology,
  • L.S. Jefferson
    Penn State College of Medicine, Milton S. Hershey Medical Center, HERSHEY, PA
    Department of Physiology,
  • S.R. Kimball
    Penn State College of Medicine, Milton S. Hershey Medical Center, HERSHEY, PA
    Department of Physiology,
  • T.W. Gardner
    Penn State College of Medicine, Milton S. Hershey Medical Center, HERSHEY, PA
    Department of Ophthalmology,
  • Penn State Retina Research Group
    Penn State College of Medicine, Milton S. Hershey Medical Center, HERSHEY, PA
  • Footnotes
    Commercial Relationships  P.E. Fort, None; K.A. Gilbert, None; C.L. Bradley, None; R.S.J. Singh, None; C.E.N. Reiter, None; L.S. Jefferson, None; S.R. Kimball, None; T.W. Gardner, None.
  • Footnotes
    Support  JDRF, ADA, NIH DK15658
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 1738. doi:
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      P.E. Fort, K.A. Gilbert, C.L. Bradley, R.S. J. Singh, C.E. N. Reiter, L.S. Jefferson, S.R. Kimball, T.W. Gardner, Penn State Retina Research Group; Normal Rat Retina Exhibits A Higher Protein Synthesis Rate Than Skeletal Muscle . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1738.

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Abstract

Purpose: : To investigate the regulation of global protein synthesis in retina in contrast to another tissue that has been extensively studied.

Methods: : Global rates of protein synthesis in retina and gastrocnemus muscle were assessed in normal rats by measuring the incorporation of H3–phenylalanine into protein following intravenous administration of a flooding dose of the radiolabeled amino acid. In addition, the RNA content and the relative expression of actin and cyclophilin A were assessed by real–time PCR.

Results: : The global rate of protein synthesis in the retina was approximately 3 fold higher than that in gastrocnemius muscle and was correlated with a higher content of RNA/µg of protein and a greater relative expression of the mRNAs for the housekeeping genes, actin and cyclophilin A.

Conclusions: : Retina exhibits a high global protein synthesis rate and a high rate of gene transcription, findings that are consistent the high metabolic activity of this tissue. Understanding the basic mechanisms of retinal protein synthesis regulation is a key in the comprehension of the retinal disease as the diabetic retinopathy.

Keywords: diabetes • transcription • retina: neurochemistry 
© 2006, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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