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P.E. Fort, K.A. Gilbert, C.L. Bradley, R.S. J. Singh, C.E. N. Reiter, L.S. Jefferson, S.R. Kimball, T.W. Gardner, Penn State Retina Research Group; Normal Rat Retina Exhibits A Higher Protein Synthesis Rate Than Skeletal Muscle . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1738.
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To investigate the regulation of global protein synthesis in retina in contrast to another tissue that has been extensively studied.
Global rates of protein synthesis in retina and gastrocnemus muscle were assessed in normal rats by measuring the incorporation of H3–phenylalanine into protein following intravenous administration of a flooding dose of the radiolabeled amino acid. In addition, the RNA content and the relative expression of actin and cyclophilin A were assessed by real–time PCR.
The global rate of protein synthesis in the retina was approximately 3 fold higher than that in gastrocnemius muscle and was correlated with a higher content of RNA/µg of protein and a greater relative expression of the mRNAs for the housekeeping genes, actin and cyclophilin A.
Retina exhibits a high global protein synthesis rate and a high rate of gene transcription, findings that are consistent the high metabolic activity of this tissue. Understanding the basic mechanisms of retinal protein synthesis regulation is a key in the comprehension of the retinal disease as the diabetic retinopathy.
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