May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Intravitreal Administration of VEGF Trap Inhibits Pathological Retinal Neovascularization in a Mouse Model of Oxygen Induced Retinopathy
Author Affiliations & Notes
  • R.A. Renard
    Eye Research, Regeneron Pharmaceuticals Inc., Tarrytown, NY
  • I.B. Lobov
    Eye Research, Regeneron Pharmaceuticals Inc., Tarrytown, NY
  • Y. Liu
    Eye Research, Regeneron Pharmaceuticals Inc., Tarrytown, NY
  • J. Cao
    Eye Research, Regeneron Pharmaceuticals Inc., Tarrytown, NY
  • K.D. Anderson
    Eye Research, Regeneron Pharmaceuticals Inc., Tarrytown, NY
  • N.J. Papadopoulos
    Eye Research, Regeneron Pharmaceuticals Inc., Tarrytown, NY
  • J.S. Rudge
    Eye Research, Regeneron Pharmaceuticals Inc., Tarrytown, NY
  • G.D. Yancopoulos
    Eye Research, Regeneron Pharmaceuticals Inc., Tarrytown, NY
  • S.J. Wiegand
    Eye Research, Regeneron Pharmaceuticals Inc., Tarrytown, NY
  • Footnotes
    Commercial Relationships  R.A. Renard, Regeneron Pharmaceuticals Inc., E; I.B. Lobov, Regeneron Pharmaceuticals Inc., E; Y. Liu, Regeneron Pharmaceuticals Inc., E; J. Cao, Regeneron Pharmaceuticals Inc., E; K.D. Anderson, Regeneron Pharmaceuticals Inc., E; N.J. Papadopoulos, Regeneron Pharmaceuticals Inc., E; J.S. Rudge, Regeneron Pharmaceuticals Inc., E; G.D. Yancopoulos, Regeneron Pharmaceuticals Inc., E; S.J. Wiegand, Regeneron Pharmaceuticals Inc., E.
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 1750. doi:
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      R.A. Renard, I.B. Lobov, Y. Liu, J. Cao, K.D. Anderson, N.J. Papadopoulos, J.S. Rudge, G.D. Yancopoulos, S.J. Wiegand; Intravitreal Administration of VEGF Trap Inhibits Pathological Retinal Neovascularization in a Mouse Model of Oxygen Induced Retinopathy . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1750.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : In the Oxygen–Induced Retinopathy (OIR) model, hyperoxia induces vaso–obliteration in the central retina of neonatal mice followed by ischemia–induced pathological retinal neovascularization when animals are returned to room air. We have previously reported that systemic administration of VEGF Trap, a potent VEGF inhibitor which binds all VEGF–A isoforms and placental growth factor, blocks pathological neovascularization in this model (Invest. Ophthalmol. Vis. Sci. 2002 43: E–Abstract 3714). The present studies assess the effects of intravitreal administration of VEGF Trap on pathological retinal neovascularization in OIR.

Methods: : OIR was produced following the method developed by Smith et al (Invest. Ophthalmol. Vis. Sci. 1994, 35:101–111). To assess the effect of intravitreal delivery, VEGF Trap or a control protein, hFc, was injected intravitreally (ITV) on P14, 2 days following return to room air. Eyes were enucleated at P17, and one retina was flat–mounted and stained with FITC –labeled Griffonia Simplicifolia Lectin I to visualize the retinal vasculature. The contralateral eye was embedded, sectioned and stained with hematoxylin and eosin.

Results: : Within five days following return to room air at P17, the retinas of all control mice exhibited marked pathological angiogenesis, characterized by the presence of vascular tufts penetrating the inner limiting membrane and chaotic sprouting of vessels on the surface of the retina. Intravitreal administration of VEGF Trap almost completely blocked the development of these vascular abnormalities.

Conclusions: : Intravitreal administration of VEGF Trap efficiently suppresses pathologic retinal angiogenesis.

Keywords: neovascularization • retinopathy of prematurity • hypoxia 
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