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Y. Yang, Y. Yu; The Differentiation Potential of Rat Bone Marrow Mesenchymal Stem Cells Into Retina in Laser–Injured Rat Eye . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1774.
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© ARVO (1962-2015); The Authors (2016-present)
To observe the differentiation potential of SD rat bone marrow mesenchymal stem cells (MSCs) which were subretinally transplanted into laser–injured retina in vivo without differentiation inducements in vitro.
SD rat MSCs were cultivated in vitro to passage 3 and were injected into the sub–retina space of laser–injured rat retina with Bromo–Deoxyuridine (BrdU) labeling to trace the origin of the repopulating cells. After 4 weeks, eyeballs were enucleated for continuous paraffin slides. The expressions of Nestin, NeuN, GFAP, Vimentin, Rhodopsin were detected by immunofluorescence.
BrdU–positive MSCs were present in mutiple locations, including photoreceptor layer, the bipolar layers and the ganglion cell layer. These cells expressed Nestin, NeuN, GFAP, Vimentin, and Rhodopsin.
For SD rats, transplanted MSCs in injured retina can be integrated into the host retina and partially differentiate into nerve cells and photoreceptors in vivo.
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