May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
DAF and CD59 Are Essential to Suppress T Cell Responses in Corneal Transplantation
Author Affiliations & Notes
  • A.A. Esposito
    Case Western Reserve University, Cleveland, OH
    Ophthalmology,
  • B. Suedekum
    Case Western Reserve University, Cleveland, OH
    Ophthalmology,
  • J. Liu
    Case Western Reserve University, Cleveland, OH
    Pathology,
  • J. Lass
    Case Western Reserve University, Cleveland, OH
    Ophthalmology,
  • F. Lin
    Case Western Reserve University, Cleveland, OH
    Pathology,
  • M.E. Medof
    Case Western Reserve University, Cleveland, OH
    Ophthalmology,
    Pathology,
  • Footnotes
    Commercial Relationships  A.A. Esposito, None; B. Suedekum, None; J. Liu, None; J. Lass, None; F. Lin, None; M.E. Medof, None.
  • Footnotes
    Support  NIH grant EY11288
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 1782. doi:
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      A.A. Esposito, B. Suedekum, J. Liu, J. Lass, F. Lin, M.E. Medof; DAF and CD59 Are Essential to Suppress T Cell Responses in Corneal Transplantation . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1782.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : In recent studies, we showed that DAF and CD59, originally characterized as cell surface regulators which protect self cells from complement attack, play a central role in the induction of T cell response. In other work, we showed that the two molecules are essential for anterior chamber–associated immune deviation (ACAID). To study their importance in suppressing T cell responses against corneal transplants, we measured T cell anti–male Dby and Uty responses following transplantation of male corneas from Daf1–/–CD59a–/– mice or WTs to female WT recipients, and male corneas from Daf1–/–CD59a–/– mice or WTs to female Daf1–/–CD59a–/– recipients.

Methods: : 2 mm corneal grafts from each of the mouse groups were transplanted using 8 interrupted sutures into 1.5 mm beds. All animals were mixed 129/C57BL/6 backcrossed 5 generations on the C57BL/6 background. Following transplantation, rejection was graded clinically, histology analyzed, and recipient CD4 and CD8 responses to male antigen measured by IFN–γ ELISPOTS to Dby and Uty respectively.

Results: : Marked responses to both CD4–restricted Dby and CD8–restricted Uty were seen in Daf1–/– transplants and Daf1–/–CD59a–/– corneas. Similarly increased results were found when WT male corneas were used and when recipients were deficient in DAF or both regulators.

Conclusions: : DAF and CD59 on both transplants and recipients are necessary to suppress both CD4 and CD8 responses to corneal transplantation. The results suggest that administration of DAF and/or CD59 could have clinical value in high risk transplants.

Keywords: cornea: basic science • immunomodulation/immunoregulation • inflammation 
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