May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Aquaporin Gene Expression in Trabecular Meshwork Cells
Author Affiliations & Notes
  • A. Ando
    Ophthalmology, Kansai Medical University, Moriguchi, Japan
  • N. Kiriyama
    Ophthalmology, Kansai Medical University, Moriguchi, Japan
  • S. Kaneko
    Ophthalmology, Kansai Medical University, Moriguchi, Japan
  • T. Nishimura
    Ophthalmology, Kansai Medical University, Moriguchi, Japan
  • M. Matsumura
    Ophthalmology, Kansai Medical University, Moriguchi, Japan
  • Footnotes
    Commercial Relationships  A. Ando, None; N. Kiriyama, None; S. Kaneko, None; T. Nishimura, None; M. Matsumura, None.
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 1841. doi:
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    • Get Citation

      A. Ando, N. Kiriyama, S. Kaneko, T. Nishimura, M. Matsumura; Aquaporin Gene Expression in Trabecular Meshwork Cells . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1841.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : We examined the gene expression of aquaporin (AQP), a water channel protein, and of the ion channel proteins Kir 7.1, Kir 2.1, and Na+/K+ ATPase, in human and porcine trabecular meshwork (TM) cells.

Methods: : TM cells were obtained from a porcine eye, while human TM cells were obtained from a patient with primary open angle glaucoma during a trabeculectomy. The cells were cultured in F12/DME medium with 5% fetal bovine serum added and used at the second to third passage. Total RNA was extracted and reverse transcribed into cDNA. A reverse–transcribed polymerase chain reaction (RT–PCR) method was performed to detect the gene expression of AQP–1 through AQP–9, Kir 7.1, Kir 2.1, and Na+/K+ ATPase using specific primers for the cDNA sequences.

Results: : Among the molecules examined, the gene expression of AQP–1, AQP–2, AQP–3, AQP–4, AQP–5, Kir 7.1, Kir 2.1, and Na+/K+ ATPase was detectable, while the others showed only faint evidence following amplification by RT–PCR.

Conclusions: : We considered that these molecules may play important roles in the regulatory system for intraocular pressure by controlling aqueous outflow and ion transport.

Keywords: trabecular meshwork • aqueous • gene/expression 
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