May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Characterization of the Aqueous Outflow Pathway and Expression of Endothelial Leukocyte Adhesion Molecule 1 (ELAM–1) in Glaucoma–Induced Porcine Eyes
Author Affiliations & Notes
  • E. Vecino
    Cell Biology & Histology, University of the Basque Country, Leioa, Spain
  • T. Suárez
    Cell Biology & Histology, University of the Basque Country, Leioa, Spain
  • M. Hernández
    Cell Biology & Histology, University of the Basque Country, Leioa, Spain
  • J.H. Urcola
    Cell Biology & Histology, University of the Basque Country, Leioa, Spain
    Hospital of Mendaro, Guipuzcoa, Spain
  • D. Rodriguez
    Biochemistry, University of Salamanca, Salamanca, Spain
  • Footnotes
    Commercial Relationships  E. Vecino, None; T. Suárez, None; M. Hernández, None; J.H. Urcola, None; D. Rodriguez, None.
  • Footnotes
    Support  ONCE, Fundaluce, The American Glaucoma Foundation, MCYT (BFI2003–07177), University of the Basque Country (00077.327–15350/2003) and Gangoiti Foundation.
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 1851. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      E. Vecino, T. Suárez, M. Hernández, J.H. Urcola, D. Rodriguez; Characterization of the Aqueous Outflow Pathway and Expression of Endothelial Leukocyte Adhesion Molecule 1 (ELAM–1) in Glaucoma–Induced Porcine Eyes . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1851.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To characterize the aqueous outflow pathway of the pig eye and to determine whether the expression of ELAM–1 in the trabecular meshwork system of the porcine eye subjected to induced glaucoma is elevated, as naturally occurs in human glaucoma.

Methods: : Fluorescent dextrans and microspheres were applied to the anterior chamber of the porcine eye in order to identify the outflow pathway. Twenty to sixty minutes after application of the dextrans and a few days to months after application of the microspheres, pig eyes were fixed with 4% paraformaldehyde, cryoprotected, OCT embedded and sectioned. The localization of the fluorescent dextrans and microspheres was identified by fluorescent microscopy. Moreover ELAM–1 was immunohistochemically detected in the trabecular meshwork system of 5 pigs, in which cauterization of the episcleral veins was performed to the left eyes to induce glaucoma, while the right eyes were maintained intact as controls.

Results: : The aqueous outflow pathway of the porcine eye is principally made up of a series of endothelial–cell–lined multiple channels in the angle of the anterior chamber comprising the trabecular meshwork and Schlemm’s canals, which are connected to the episcleral venous system. Immunodetecion of ELAM–1 in a human trabecular sample obtained from trabeculectomy was performed as a positive control. ELAM–1 expression was found to be moderately elevated in the trabecular meshwork of the glaucomatous porcine eye and not present in that of the control eye.

Conclusions: : We have characterized the trabecular meshwork and multiple Schlemm's canals in the porcine eye. Our results indicate that overexpression of ELAM–1 in porcine eyes with induced glaucoma is related to increased IOP, as well as retinal ganglion cell loss, and optic nerve damage.

Keywords: outflow: trabecular meshwork • intraocular pressure • anterior chamber 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×