May 2006
Volume 47, Issue 13
ARVO Annual Meeting Abstract  |   May 2006
Absence of PEDF in Human Tears of Healthy Individuals
Author Affiliations & Notes
  • G.B. van Setten
    Ophthalmology, Karolinska, Stockholm, Sweden
  • O. Abdiu
    Ophthalmology, Karolinska, Stockholm, Sweden
  • G. van Setten
    Ophthalmology, Karolinska, Stockholm, Sweden
  • Footnotes
    Commercial Relationships  G.B. van Setten, None; O. Abdiu, None; G. van Setten, None.
  • Footnotes
    Support  Synfrämjandets Forskningsfond Stockholm , Sweden
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 1967. doi:
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      G.B. van Setten, O. Abdiu, G. van Setten; Absence of PEDF in Human Tears of Healthy Individuals . Invest. Ophthalmol. Vis. Sci. 2006;47(13):1967.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To investigate if PEDF as secretory protein and component of human corrneal epithelium does occur in tear fluid of human healthy individuals in measurable concentrations.

Methods: : Tear fluid samples prior and after stimulation were collected with blunted capillaries as reported before from 20 healthy individuals from both eyes. Due to the very little volume of individual samples collected prior to stimulation even pooled samples of unstimualted tears were created and analyzed. An ELISA assay, specific for PEDF was used for the analysis. Samples were analysed in duplicate whenever possible. As control served human recombinant PEDF.

Results: : All samples turned out to be negative within the detection range. Even the pooled samples collected prior to induction of reflex tearing did not reveal the presence of any significant amount of PEDF.

Conclusions: : The presence of PEDF in superficial layers of ocular surface tissues such as conjunctiva and cornea does most likely reflect a local production of PEDF in these tissues. The hypothesis that the PEDF localized in these tissues could derive from tear fluid can, according to our preliminary results, not be supported.

Keywords: cornea: tears/tear film/dry eye • lacrimal gland • cytokines/chemokines 

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