May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Will the Fortification of Optisol GS® With Voriconazole Reduce the Fungal Contamination Rate of Corneal Donor Rim Cultures?
Author Affiliations & Notes
  • M.K. Shah
    New York Eye and Ear Infirmary, New York, NY
    Pathology,
  • D.C. Ritterband
    New York Eye and Ear Infirmary, New York, NY
    Ophthalmology,
  • R. Yang
    New York Eye and Ear Infirmary, New York, NY
    Ophthalmology,
  • S.W. Meskin
    New York Eye and Ear Infirmary, New York, NY
    Ophthalmology,
  • J.A. Seedor
    New York Eye and Ear Infirmary, New York, NY
    Ophthalmology,
  • A. Bhargava
    New York Eye and Ear Infirmary, New York, NY
    Ophthalmology,
  • R.S. Koplin
    New York Eye and Ear Infirmary, New York, NY
    Ophthalmology,
  • Footnotes
    Commercial Relationships  M.K. Shah, None; D.C. Ritterband, None; R. Yang, None; S.W. Meskin, None; J.A. Seedor, None; A. Bhargava, None; R.S. Koplin, None.
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 2351. doi:
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      M.K. Shah, D.C. Ritterband, R. Yang, S.W. Meskin, J.A. Seedor, A. Bhargava, R.S. Koplin; Will the Fortification of Optisol GS® With Voriconazole Reduce the Fungal Contamination Rate of Corneal Donor Rim Cultures? . Invest. Ophthalmol. Vis. Sci. 2006;47(13):2351.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To assess the efficacy of voriconazole as an additive to Optisol GS® (gentamicin 100 micrograms/ml and streptomycin 200 micrograms/ml) in reducing fungal corneal donor rim contamination.

Methods: : 302 consecutive donor corneal rims stored in Optisol GS® and used in penetrating keratoplasty were bisected under sterile conditions by the operating surgeon and placed back in the storage medium. Two separate preparations of "fresh" Optisol GS® solution were prepared for each half donor rim. One preparation (Tube A) contained Optisol GS® while the other (Tube B) contained Optisol GS® fortified with 100 micrograms/ml of voriconazole. Tube A and Tube B were refrigerated at 3ºC for 24 hours. The rims were then removed and placed in thioglycolate broth (BBL) and incubated at 37º C for 7 days. Turbidity was assessed daily. If turbidity was noted, gram stain and subculture on sabouraud dextrose agar was performed.

Results: : The rate of culture positive donor rims in the Optisol GS® (Tube A) was 14.2% (43/302). The rate of positive cultures in the Optisol GS® with voriconazole (Tube B) was 12.6% (38/302). 38 bacteria grew in both media (P.acnes 11, S. epidermidis 14, S. viridans 6, S. aureus 4, P. aeruginosa 2, Serratia marcesans 1 .Five fungal organisms (C. parapsilosis 2, C. albicans 2, C. famata 1) grew in the standard Optisol GS® media (Tube A). No fungal organisms grew in Optisol GS® with voriconazole (Tube B).

Conclusions: : The addition of 100micrograms/ml of voriconazole to Optisol GS® appears to reduce the rate of fungal growth in corneal rims stored in Optisol GS®.

Keywords: cornea: storage • fungal disease • drug toxicity/drug effects 
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